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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,000 results for: sgRNA

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Showing: 7681 - 7700 of 9000 results

  1. pSJX023

    Plasmid
    #232326
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Promoter
    PJ23119 and Pxyl
    Available Since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    232326-plasmid-map-sequence-id-496249

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  6. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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  7. pLCKO2

    Plasmid
    #125518
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125518-plasmid-map-sequence-id-248252

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  9. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171993-plasmid-map-sequence-id-340552

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  12. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid
    #207773
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207773-plasmid-map-sequence-id-413797

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  18. pGES201

    Plasmid
    #190197
    Purpose
    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection
    Depositor
    Yuefeng Guan
    Article
    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available Since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190197-plasmid-map-sequence-id-371938

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  19. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

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Showing: 7681 - 7700 of 9000 results