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TERT

Plasmid

#156078

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TERT (TERT Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L861Q)

Plasmid

#32068

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L861Q

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabe EGFR(L861Q)

Plasmid

#32068

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L861Q

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBrain-GFP-FKBP-TACC3-shTACC3

Plasmid

#59354

Purpose

Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

EGFP and FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-FKBP-TACC3-shTACC3

Plasmid

#59354

Purpose

Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

EGFP and FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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PPIB

Plasmid

#155838

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPIB (PPIB Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PPIB

Plasmid

#155838

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPIB (PPIB Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF4 CMV 5TOP intron renilla TRICK CTE polyA

Plasmid

#64544

Purpose

5′ TOP TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, 5' TOP (5′ terminal oligo…

Expression

Mammalian

Promoter

Tetracycline-inducible promoter and 5′ UTR of hum…

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV 5TOP intron renilla TRICK CTE polyA

Plasmid

#64544

Purpose

5′ TOP TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, 5' TOP (5′ terminal oligo…

Expression

Mammalian

Promoter

Tetracycline-inducible promoter and 5′ UTR of hum…

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Article

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Expression

Bacterial

Promoter

trcp

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Article

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Expression

Bacterial

Promoter

trcp

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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FUT8-Dox

Plasmid

#124631

Purpose

Doxycycline-inducible FUT8 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Alpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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FUT8-Dox

Plasmid

#124631

Purpose

Doxycycline-inducible FUT8 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Alpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Dll4ICD-HPC4 pBacPAK8.3

Plasmid

#17335

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 4 (Dll4 Mouse)

Tags

HPC4

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Dll4ICD-HPC4 pBacPAK8.3

Plasmid

#17335

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 4 (Dll4 Mouse)

Tags

HPC4

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

Plasmid

#214880

Purpose

Lentiviral vector encoding RfxCas13d targeting CD46 guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crCD46-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

Plasmid

#214880

Purpose

Lentiviral vector encoding RfxCas13d targeting CD46 guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crCD46-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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EE-LRRK2

Plasmid

#193583

Purpose

Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

FYVE-CC2 and 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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EE-LRRK2

Plasmid

#193583

Purpose

Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

FYVE-CC2 and 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAdTrack-CMV HA ALAS-1

Plasmid

#22746

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

ALAS-1 (Alas1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only