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Plasmid

#239002

Purpose

Final circuit with -Ara: OFF, +Ara: ON behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression only in the presence of Ara (-Ara: OFF, +Ara: ON)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Hygro-hTNNT2-FUUCIplex

Plasmid

#240220

Purpose

Multiplexable FUCCI sensor (FUCCIplex). The hTNNT2 promoter drives the expression of the fluorescent sensor.

Depositor

Francesco Pasqualini

Article

Di Sante et al bioRxiv 2024.12.19.629259

Insert

FUCCIplex

Use

Lentiviral

Expression

Mammalian

Promoter

hTNNT2

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisPNK

Plasmid

#216670

Purpose

Expresses his-tagged PNK in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 Polynucleotide Kinase (pseT T4 phage)

Tags

his-tag

Expression

Bacterial

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisM5RT

Plasmid

#216672

Purpose

Expresses his-tagged Mut5 reverse transcriptase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

thermostable M-MuLV reverse transcriptase variants

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJL178

Plasmid

#243687

Purpose

Stably expresses fusion of SUPV3L1(1-114aa) and ALDH18A1(106-795aa)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of SUPV3L1-(1-114aa)-ALDH18A1(106-795aa)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pREA3

Plasmid

#245832

Purpose

Express rac-2(G12V) in all neurons.

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rac-2(G12V) (rac-2 Nematode)

Expression

Worm

Mutation

G12V

Promoter

rgef-1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201_PARG WT

Plasmid

#240315

Purpose

Entry vector for Gateway with PARG

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARG (PARG Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pJH105

Plasmid

#243726

Purpose

Expresses PMPCA

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

PMPCA (PMPCA Human)

Use

Lentiviral

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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CD_bsd

Plasmid

#139945

Purpose

Resistance gene

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

blasticidin-S deaminase

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CD_nat

Plasmid

#139946

Purpose

Resistance gene

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

nourseothricin acetyltransferase

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CF_OriT

Plasmid

#139948

Purpose

Origin of transfer for conjugation cargo plasmids

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

OriT

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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EF_tTpEF2

Plasmid

#139951

Purpose

Diatom terminator

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thalassiosira pseudonana EF2 terminator

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AC_pTpEF2

Plasmid

#139938

Purpose

Diatom promoter

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thalassiosira pseudonana EF2 promoter

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJFT7_nHalo_DC(r4)_PARG_Mutation

Plasmid

#240316

Purpose

Gateway compatible vector expressing PARG (E755/756A)

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARG (PARG Human)

Use

Unspecified

Mutation

E755/756 A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-TurboID

Plasmid

#245241

Purpose

Proximity-labeling proteomics negative control

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

TurboID

Expression

Mammalian

Mutation

wild-type

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pREA12

Plasmid

#245770

Purpose

Allows for mCherry::RBD::T2A::rac-2::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

mCherry::RBD::T2A::rac-2::mGFP

Use

Unspecified

Tags

mCherry and mGFP

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pREA16

Plasmid

#245771

Purpose

Expresses mCherry::RBD::T2A::rac-2::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

mCherry::RBD::T2A::rac-2::mGFP

Tags

mCherry and mGFP

Expression

Worm

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSJC321

Plasmid

#245777

Purpose

Expresses rac-2(G12V)::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rac-2(G12V)::mGFP

Tags

mGFP

Expression

Worm

Mutation

G12V

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSJC319

Plasmid

#245775

Purpose

Expresses rap-1(G12V)::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rap-1(G12V)::mGFP

Tags

mGFP

Expression

Worm

Mutation

G12V

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFABs9

Plasmid

#241905

Purpose

Ferrulic acid biosensor - expresses sfGFP in response to substituted cinnamic acid

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

sfGFP, ferC, ferA

Use

Synthetic Biology

Mutation

none

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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