We narrowed to 64,663 results for: cell;

1. Cre Stoplight 2.4

   Plasmid

   #37402

   Purpose

   Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

   Depositor

   Thomas Hughes

   Article

   Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

   Insert

   Cre Stoplight 2.4
   

   Tags

   ZsGreen1 and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. mScarlet-Rab9a

   Plasmid

   #169074

   Purpose

   Express human Rab9a in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rab9a (RAB9A Human)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2_NBT:Cas9green; erbb2_gRNA171

   Plasmid

   #199337

   Purpose

   pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   NBT
   Cas9
   erbb2 sgRNA
   

   Use

   Tol2 destination vector

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMPRA2-CMV-Clover

   Plasmid

   #53539

   Purpose

   Fluorescent reporter control for pMPRA2

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   Clover GFP
   

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMPRA3-CMV-Clover

   Plasmid

   #53540

   Purpose

   Fluorescent reporter control for pMPRA3

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   Clover GFP
   

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LAMP1-GFP

   Plasmid

   #16290

   Depositor

   Ron Vale

   Article

   Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

   Insert

   LAMP1/RE72002
   

   Tags

   EGFP

   Expression

   Insect

   Promoter

   Ac5

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJC7

   Plasmid

   #177872

   Purpose

   Co-express in mammalian cells two target proteins, using an internal ribosome entry site and two multi-cloning sites.

   Depositor

   Roberto Dominguez

   Article

   Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LifeAct-mGarnet

   Plasmid

   #104307

   Purpose

   codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   LifeAct-mGarnet
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV5S-F30-2xdBroccoli

   Plasmid

   #66845

   Purpose

   Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Tags

   5S rRNA

   Expression

   Mammalian

   Promoter

   5S

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-PERK(LD)-EGFP

    Plasmid

    #225680

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xMARS

    Plasmid

    #205233

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    

    Tags

    Nuclear Export Sequence and mScarlet-i

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSNAP-macroH2A1.2 Zeo

    Plasmid

    #132428

    Purpose

    Expressing SNAP-tagged histone macroH2A1.2 in mammalian cells

    Depositor

    John M. Greally

    Article

    Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

    Insert

    macroH2A1.2
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL-MHCK7-R-GECO

    Plasmid

    #109374

    Purpose

    Expresses calcium indicator R-GECO in muscle cells

    Depositor

    Nenad Bursac

    Article

    Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

    Insert

    R-GECO
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO APEX2-GFP

    Plasmid

    #129640

    Purpose

    Express APEX2-GFP fusion in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    APEX2-GFP
    

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet1-sgRNA(siteA)

    Plasmid

    #154831

    Purpose

    sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    sgRNA(siteA)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AbVec2.1-IGLC2-MscI

    Plasmid

    #80797

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2009 Oct 31;350(1-2):

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-survivin

    Plasmid

    #196908

    Purpose

    visualization of midzone on anaphase and midbody later during mitosis in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biotechniques. 2004 Oct;37(4):552, 554,

    Insert

    survivin
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E129K

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pci-EGFP-UPF1-DEAA

    Plasmid

    #135996

    Purpose

    DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    DE647AA

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG244 iFAST

    Plasmid

    #130809

    Purpose

    Expresses iFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

    Insert

    iFAST
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. zCREST3:redCas9; control_gRNA

    Plasmid

    #199334

    Purpose

    pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    control sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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