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pRBBm69

Plasmid

#48116

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - sacB

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pLSSmKate1-Tubulin-C1

Plasmid

#31902

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

alpha-tubulin

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/mClover/nat

Plasmid

#120330

Purpose

Bacterial mClover labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Tags

D103N (see comment below)

Expression

Bacterial

Promoter

(None)

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-OX

Plasmid

#184615

Purpose

For expression of a fluorescent sensor for orexin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OX

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL13001

Plasmid

#50265

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb) + 5'UTR, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN01*-His

Plasmid

#84509

Purpose

E.coli version of EPN01*

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01*-His_pET29b

Tags

His tag

Expression

Bacterial

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP12068

Plasmid

#222356

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and kanR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFH55

Plasmid

#125814

Purpose

Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nSpCas9-PmCDA, Arabidopsis codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01

Plasmid

#80046

Purpose

expression of EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Tags

Myc

Expression

Mammalian

Mutation

M1I

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHCMV_BRV_GP

Plasmid

#207258

Purpose

Mammalian expression of Blue River virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BRV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt10b

Plasmid

#42289

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt10b (Wnt10b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt8a

Plasmid

#42284

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8a (Wnt8a Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pAWp9

Plasmid

#73851

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-M.accABCD

Plasmid

#66865

Purpose

Encodes accA, accB, accC, accD in monocistronic configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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Chx10 (2.5kb distal 5' reg)+SV40p-tdTomato-IRES-AP

Plasmid

#18814

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Chx10 (2.5kb distal 5' reg)+SV40p-tdTomato-IRES-AP

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pXL-CAG-ICeuI-sHRPaNRX3beta

Plasmid

#79910

Purpose

piggyBac transposon vector, sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPaNRX3beta

Use

Piggybac

Expression

Mammalian

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRVdG-N-P-M-EGFP-SynPhRFP-L

Plasmid

#52483

Purpose

Expresses EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E mOrange + link c2

Plasmid

#54421

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + mOrange

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-MCS1M(1)Kana-mCMV-TK-pA

Plasmid

#22060

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

TK

Expression

Mammalian

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mNeonGreen

Plasmid

#191323

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mNeonGreen (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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