163,819 results

1. pPICZaa-STE2-cohis

   Plasmid

   #133433

   Purpose

   Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

   Depositor

   Gerhard Wagner

   Insert

   STE2 (STE2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C59S/C252A, Codon optimized for P. pastoris, and …

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0335

   Plasmid

   #144358

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF91 (ZNF91 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P29

   Plasmid

   #120040

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2A-NLS-tdtomato-EPG backbone

   Plasmid

   #215548

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. M77 pIRESpuro-GLUE-hDsh3

   Plasmid

   #15102

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   Dsh3 (DVL3 Human)
   

   Use

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MS2-HA-HshnRNPH1_Z

   Plasmid

   #148120

   Purpose

   Mammalian Expression of HshnRNPH1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPH1 (HNRNPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-ERG-R367K

   Plasmid

   #118624

   Purpose

   Expresses V5 tagged ERG R367K (DNA binding mutant) protein

   Depositor

   William Hahn

   Article

   Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

   Insert

   ERG (R367K DNA binding mutant) (ERG Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R367K

   Promoter

   EF1a

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT85_P438-mEOS3.2_GentaR

   Plasmid

   #173894

   Purpose

   Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

   Depositor

   Yonathan Arfi

   Article

   Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

   Insert

   mEos3.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P438

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTSlb-wt

   Plasmid

   #60728

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2xFlag CMV2-YAP2-1st WW mutant

    Plasmid

    #19046

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein isoform 3 (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine,and Proline 202…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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