161,472 results

1. pLenti6.2-3xFLAG-V5-ccdB

   Plasmid

   #87072

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

   Depositor

   Susan Lindquist, Mikko Taipale

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-PGK-hESRRB

   Plasmid

   #60434

   Purpose

   Over-expression of human ESRRB

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   ESRRB (ESRRB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-SpRY-NLS-6xHis (gbr2101)

   Plasmid

   #181743

   Purpose

   bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   bacterial codon optimized SpRY
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS(SV40)-6xHis and MKIEE MBP tag

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRosetta

   Plasmid

   #59700

   Purpose

   Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

   Depositor

   John Doench, David Root

   Inserts

   Puro
   BlastR
   EGFP
   

   Use

   Lentiviral

   Tags

   2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Integrin-Beta1-N-18

   Plasmid

   #55064

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Integrin-Beta1 (ITGB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6.2_mCherry_mito Grx1-roGFP2

   Plasmid

   #155044

   Purpose

   Fluorescent redox reporter

   Depositor

   Vanessa LaPointe

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   mitochondrial targeting sequence and roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NPY-td-Orange2

   Plasmid

   #83497

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   tandem mOrange2

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cilantro 2

   Plasmid

   #74450

   Purpose

   Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-sh1368 knockdown c-myc

    Plasmid

    #29435

    Depositor

    Bob Eisenman

    Article

    Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

    Insert

    c-Myc shRNA (MYC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP and WPRE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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