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We narrowed to 13,830 results for: cas9

Showing: 761 - 780 of 13830 results

  6. ADEPT-pCas9

    Plasmid
    #238032
    Purpose
    Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    spCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Promoter
    tet
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    238032-plasmid-map-sequence-id-475985

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  7. pQdCas9-sggfp

    Plasmid
    #236184
    Purpose
    The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Use
    Tags
    Expression
    Mutation
    Promoter
    Quorum sensing promoter
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    236184-plasmid-map-sequence-id-470109

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  8. pSEVA47_dCas9-GFP_NOT_iC

    Plasmid
    #231418
    Purpose
    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)
    Depositor
    Alfonso Jaramillo
    Article
    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev
    Inserts
    dCas9
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    231418-plasmid-map-sequence-id-468903

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  9. pdCas9-tdTomato

    Plasmid
    #232631
    Purpose
    catalytically inactive dCas9 for localization; labeled with tdTomato
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232631-plasmid-map-sequence-id-462150

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  10. pSEVA47_dCas9-GFP_AAN_4

    Plasmid
    #231415
    Purpose
    This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.
    Depositor
    Manish Kushwaha
    Article
    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.
    Inserts
    dCas9
    GFP
    sgRNA-X
    sgRNA-Y
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231415-plasmid-map-sequence-id-461754

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  15. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Article
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Use
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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  16. SpCas9-miniCGBE1

    Plasmid
    #198548
    Purpose
    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198548-plasmid-map-sequence-id-388891

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  17. SpCas9-NG-miniCGBE1

    Plasmid
    #198538
    Purpose
    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198538-plasmid-map-sequence-id-388888

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  18. SpCas9-NRRH

    Plasmid
    #198540
    Purpose
    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRRH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198540-plasmid-map-sequence-id-388897

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  19. SpCas9-NRCH

    Plasmid
    #198542
    Purpose
    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198542-plasmid-map-sequence-id-388906

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  20. DG SaCas9 GFP V3

    Plasmid
    #226961
    Purpose
    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226961-plasmid-map-sequence-id-450824

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Showing: 761 - 780 of 13830 results