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pGEL062

Plasmid

#124894

Purpose

For genome editing in plant by SpCas9 wild type

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL065

Plasmid

#124896

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NGv1)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ255

Plasmid

#124310

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A-UGI

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ255-x3.7

Plasmid

#124311

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A_x3.7-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ256-x3.7

Plasmid

#124313

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A_x3.7-PmCDA1-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ULK1-ADA

Plasmid

#249534

Purpose

Expressing human full length ULK1 with P433A/V434D/P435A mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

Flag-tag and strep-tag

Expression

Mammalian

Mutation

P433A, V434D and P435A

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-1-835-mcherry

Plasmid

#249729

Purpose

Expressing human ULK1-1-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-1-290-mcherrry

Plasmid

#249732

Purpose

Expressing human ULK1-1-290 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-290

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-290-835-mcherry

Plasmid

#249733

Purpose

Expressing human ULK1-290-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

290-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-ATG13-1-230

Plasmid

#250343

Purpose

Expressing human ATG13 (aa. 1-230)

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Tags

GFP

Expression

Mammalian

Mutation

only amino acids 1-230

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-ATG13-1-230(HF|DD)

Plasmid

#250344

Purpose

Expressing human ATG13 (1-230) with H214D/F215D mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Tags

GFP

Expression

Mammalian

Mutation

only amino acids 1-230 with H214D and F215D muta…

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

Plasmid

#237891

Purpose

Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

sgRNA-Cre

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3

Plasmid

#237892

Purpose

Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

sgRNA-Cre

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-thrombin-TEX264(28-313aa)

Plasmid

#227714

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

TEX264(28-313aa) (TEX264 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-thrombin-FAM134C(250-466aa)

Plasmid

#227715

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FAM134C(250-466aa) (RETREG3 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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PFN29A-DDX6

Plasmid

#240019

Purpose

Expresses human DDX6 fused to EGFP in bacterial cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6

Expression

Bacterial

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgCNOT3-1

Plasmid

#240008

Purpose

Expresses human sgCNOT3 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgCNOT3

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgDDX6-2

Plasmid

#240005

Purpose

Expresses human sgDDX6 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgDDX6

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgPABPC1-1

Plasmid

#240007

Purpose

Expresses human sgPABPC1 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgPABPC1

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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