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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 79,188 results for: rest

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Showing: 761 - 780 of 79188 results

  4. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61358-plasmid-map-sequence-id-99789

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  6. pCMV-lyso-pHluorin

    Plasmid
    #70113
    Purpose
    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification
    Depositor
    Christian Rosenmund, Benjamin Rost
    Publication
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
    Tags
    HA-Tag and pHluorin
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70113-plasmid-map-sequence-id-323570

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  11. pcDNA3-TLR7-YFP

    Plasmid
    #13022
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TLR7 (TLR7 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13022-plasmid-map-sequence-id-476228

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  15. pEP4 E02S EN2L

    Plasmid
    #20922
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28
    Depositor
    James Thomson
    Publication
    Yu et al Science. 2009 Mar 26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)
    Use
    Episomal/ebna
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20922-plasmid-map-sequence-id-9758

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  17. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Publication
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67639-plasmid-map-sequence-id-220324

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  19. tFucci(CA)5

    Plasmid
    #153521
    Purpose
    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.
    Depositor
    Atsushi Miyawaki
    Publication
    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tFucci(CA)5.
    Tags
    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)
    Expression
    Bacterial and Mammalian
    Promoter
    CAG
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    153521-plasmid-map-sequence-id-308996

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  20. FH-TET3-pEF

    Plasmid
    #49446
    Purpose
    This plasmid expresses full-length human TET3.
    Depositor
    Anjana Rao
    Publication
    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet methylcytosine dioxygenase 3 (TET3 Human)
    Tags
    Flag and HA
    Expression
    Mammalian
    Promoter
    EF-1alpha
    Available since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49446-plasmid-map-sequence-id-229537

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Showing: 761 - 780 of 79188 results