We narrowed to 22,787 results for: TES

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pcDNA3-KLF3

Plasmid

#49100

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

Insert

Krüppel-Like Factor 3(KLF3)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only
pCT-CMV-UBE3B-copGFP-Puro

Plasmid

#107342

Purpose

Expresses UBE3B fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

Ubiquitin-protein ligase E3B (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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ePB-PURO-FLAG-METTL3

Plasmid

#160252

Purpose

For stable integration of FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab4a-c-Myc

Plasmid

#79800

Purpose

Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Tags

TagRFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#47455

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Use

AAV

Tags

2A_GFP

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pINT-PuroDDHNF4a2

Plasmid

#31443

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

HNF4A (HNF4A Human)

Use

Phic31/attb

Tags

FKBP L106P

Expression

Mammalian

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-Phi3-Zsgreen-PGK-mCherry

Plasmid

#153320

Purpose

Expresses zsGreen and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST

Plasmid

#34922

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 1-393) (TP53 Human)

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX 4T1 RARalpha

Plasmid

#35557

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
myc-Atg14 (1-181)

Plasmid

#86752

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-181

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 pcDNA1 neo

Plasmid

#13585

Depositor

Dean Sheppard

Article

Yokosaki et al J Biol Chem. 1994 Oct 28. 269(43):26691-

Insert

integrin alpha 9 (ITGA9 Human)

Expression

Mammalian

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_nocharge

Plasmid

#139118

Purpose

express hnRNPA2 LC with no charged residues

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all charged residues are changed to S/Q except fo…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-Myc-CRBN-pGK-HYG

Plasmid

#107376

Purpose

Lentiviral expression of myc-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

UbcP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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HA-PRAS40 AA

Plasmid

#86759

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Tags

HA

Expression

Mammalian

Mutation

AA (S183A, S221A)

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-EZH2-T487A mutant

Plasmid

#28061

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Mutation

T487A

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pCK314

Plasmid

#110545

Purpose

Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

Inserts

Modified PrhaBAD (CRP-binding sites removed)
yfp
rhaS

Expression

Bacterial

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-PPxY

Plasmid

#61006

Purpose

Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 PPxY mutation (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

PPxY mutation

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pETM33_WDR5_WD

Plasmid

#178491

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

WDR5_WD (WDR5 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (∂393)

Plasmid

#24812

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-393. (No PDZ binding…

Available Since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-HA-TRAIL-R4

Plasmid

#61385

Purpose

Expresses HA tagged TRAIL receptor 4

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 4 (TNFRSF10D Human)

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R4 leader peptide (aa1-29) replaced with TN…

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
hTRAFD1-pCMV14-3xFlag

Plasmid

#73587

Purpose

Express human TRAFD1 (FLN29) in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

The original vector hTRAFD1-mCMV14-Flag was a gif…

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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NC3 pCDNA3.1 FLAG WTX (1-804)

Plasmid

#36958

Depositor

Randall Moon

Article

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pLKO-RFP-IKZF3-sh3

Plasmid

#69044

Purpose

3rd generation lentiviral vector expressing IKZF3 shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-FKBP51

Plasmid

#89132

Purpose

mammalian expression of FKBP5 also referred to as FKBP51 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

FKBP5 (FKBP5 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (1-200)

Plasmid

#86750

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-200

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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BMR1_01g02031-bio-His

Plasmid

#108116

Purpose

[Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag

Depositor

Gavin Wright

Article

Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

Insert

BMR1_01g02031

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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A*03:02:01

Plasmid

#203294

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*03:02:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387D)

Plasmid

#220463

Purpose

Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to aspartate

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN4

Plasmid

#52679

Purpose

shRNA against α-actinin-4 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN4 (Actn4 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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