We narrowed to 10,082 results for: crispr plasmids
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Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
H52-pUCM-hNGN2-inv
Plasmid#110492PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology armDepositorInsertsUseCRISPR, Cre/Lox, and TALENExpressionMammalianPromoterCAG, EF-1 alpha, and TRE3GAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPP444
Plasmid#158802PurposePlasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-3
ExpressionMammalianAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX552-rScn2a-3xV5-Syn-miRFP670
Plasmid#182566PurposeFor rat Nav1.2 knockin with 3xV5 at the extracellular loopDepositorInsertsrat Scn2a gRNA and 3xV5 donor DNA
miRFP670
UseAAV and CRISPRPromoterSyn and U6Available SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI
Plasmid#188703PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD283
Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-EGFP-KASH
Plasmid#154374PurposeVector for Flp-dependent expression of EGFP tagged KASH protein domainDepositorInsertEGFP-KASH
UseAAVExpressionMammalianAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS160 CRISPEY HDV-HDV NotI
Plasmid#114453PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsExpressionYeastPromoterScGAL7pAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e3
Plasmid#120341PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorInsertchr8:129056967-129058438 (hg19)
UseLuciferaseExpressionMammalianPromoterMYCAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPP394
Plasmid#158800PurposePlasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-1
ExpressionMammalianAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4
Plasmid#80601PurposeExpresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4
UseAAVExpressionMammalianPromoterCBAAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC1430
Plasmid#127981PurposeAMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mGreenLantern
Plasmid#219643PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest methodDepositorInsertmGreenLantern SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-sfGFP-deadTET1CD
Plasmid#184442PurposeCatalytically DEAD TET1CD with scFv against GCN4DepositorInsertCatalytically DEAD TET1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e5
Plasmid#120343PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorInsertchr8:130594100-130596043 (hg19)
UseLuciferaseExpressionMammalianPromoterMYCAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e4
Plasmid#120342PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorInsertchr8:129060477-129062593 (hg19)
UseLuciferaseExpressionMammalianPromoterMYCAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC3-yeGFP
Plasmid#176181Purposeyeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFPDepositorInsertyEGFP
UseSynthetic BiologyAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only