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SFFV-MXD1-Brd 16

Plasmid

#218999

Purpose

Transcription factor MXD1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

MXD1 (MXD1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-NFATC3-Brd 12

Plasmid

#218995

Purpose

Transcription factor NFATC3 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

NFATC3 (NFATC3 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-TBX21-Brd 13

Plasmid

#218996

Purpose

Transcription factor TBX21 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

TBX21 (TBX21 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-Regnase-3 WT-T2A-copGFP

Plasmid

#222660

Purpose

Lentiviral vector to express mouse Regnase-3 WT

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

mutated stop codon prior to a T2A peptide

Promoter

EF1α

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 D141N

Plasmid

#222654

Purpose

Mammalian expression vector to express mouse Regnase-1 D141N mutant

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 D252N

Plasmid

#222652

Purpose

For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

Plasmid

#222649

Purpose

For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-4_Crp

Plasmid

#217168

Purpose

Mammalian expression vector for expression of hAT-4_Crp transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-4_Crp transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-1_AMi

Plasmid

#217170

Purpose

Mammalian expression vector for expression of hAT-1_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-1_AMi transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Mariner-2_AMi

Plasmid

#217171

Purpose

Mammalian expression vector for expression of Mariner-2_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Mariner-2_AMi transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-13_AMi

Plasmid

#217172

Purpose

Mammalian expression vector for expression of hAT-13_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-13_AMi transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*/PBM*

Plasmid

#195221

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

Dual mutant for HRS>AAA / lacking DTHL

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper DGK zeta human

Plasmid

#224575

Purpose

To downregulate the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase zeta human (DGKZ Human)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK alfa mouse

Plasmid

#224576

Purpose

Negative Control for downregulation of the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Harbinger-1_Crp-Myb

Plasmid

#217219

Purpose

Mammalian expression vector for expression of transposase (CMV promoter)

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Harbinger-1_Crp myb like protein

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-FL_WobbleMut

Plasmid

#209053

Purpose

Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8_WobbleMut

Plasmid

#209054

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

Plasmid

#207136

Purpose

DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3D-COMP5AP-AviTag-9xHis

Plasmid

#157530

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3D (SEMA3D Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PSG7-COMP5AP-AviTag-9xHis

Plasmid

#157381

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG7 (PSG7 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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