We narrowed to 10,808 results for: T7
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Plasmid#149686PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCNNM3-CBS-YPet (CNNM3 Human, Synthetic)
Tags8 histidine tagExpressionBacterialPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMtb CblA
Plasmid#138298PurposeExpression of Mtb CblADepositorInsertMtb CblA
TagsHis tagExpressionBacterialPromoterT7Available SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p4973 pcDNA4-E6AP III C21Y
Plasmid#32890DepositorInsertE6AP (UBE3A Human)
Tags6 His Express and T7-tagExpressionMammalianMutationC21YPromoterCMVAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain
Plasmid#101205PurposeBacterial expression plasmid for SpCas9 REC3 domainDepositorInsertSpCas9 variant K506–Q712
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK506–Q712PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1-EPA-DNNNS-DQNRT
Plasmid#128397PurposeIn vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sitesDepositorInsertExotoxin A
Tags6xHis tagExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.0
Plasmid#139477PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
ExpressionMammalianMutationV413LPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD102
Plasmid#131403PurposeE. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD102
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_ZYMND11_coiled-coil MYND
Plasmid#178493PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)
TagsHis-GSTExpressionBacterialPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C, A92E)
Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
ExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-Fgß
Plasmid#101719Purposewildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forcesDepositorInsertddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p148
Plasmid#62616PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1-GATA4-opposite orientation
Plasmid#51928Purposefor T7 directed antisense production of mouse GATA4DepositorAvailable SinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FOXM
Plasmid#67118PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDC183
Plasmid#106415PurposeCo-expresses trans SNARE complex with pQZ210 in E. coliDepositorInsertsExpressionBacterialMutationFragment 141-204, Codon optimization and Fragment…PromoterT7Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hAREG
Plasmid#199229PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertAmphiregulin preproprotein (AREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PTK2_Focal-AT
Plasmid#178485PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGST-TEV-yVms1(1-417,delta(38-69))
Plasmid#128739PurposeBacterial overexpression and crystallizationDepositorInsertVms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
TagsGSTExpressionBacterialMutationresidues 1-417 with deletion of 38-69PromoterT7Available SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos4b-V69T
Plasmid#98572Purposephotoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertmEos4b-V69T
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only