We narrowed to 19,007 results for: REV

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5'-3xFLAG minimal CMV pCDNA5

Plasmid

#212044

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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Human RNF144B (27-236)

Plasmid

#110753

Purpose

Bacterial Expression of RNF144B RBR domain

Depositor

David Komander

Article

Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

Insert

RNF144B (RNF144B Human)

Tags

N-His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW073

Plasmid

#64754

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Hygro

Plasmid

#124301

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP mCherry-ApoB-EGFP

Plasmid

#112859

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

mCherry-apoB-EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTH847-gst-optsc

Plasmid

#96908

Purpose

Plasmid for yeast expression of GST encoded by preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

yeast codon optimised glutathione-S transferase

Tags

8xhistidine

Expression

Yeast

Promoter

TDH3

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag

Plasmid

#231594

Purpose

Express high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Kozak sequence-Tornado-10xSunTag-2xALFATag

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAT15482_ABE8e

Plasmid

#174120

Purpose

Expresses ABE8e with an EF1a promoter

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

eABE

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx2(yeast)

Plasmid

#65005

Purpose

Yeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

GRX2

Tags

roGFP2

Expression

Yeast

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pAT15068-evoCBE

Plasmid

#163013

Purpose

plasmid expressing evoCBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

evoCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP713

Plasmid

#136562

Purpose

F-actin labeling with a near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LifeAct-miRFP713

Tags

miRFP713

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt2

Plasmid

#184503

Purpose

Mammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 2 (Syt2 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCPP5741

Plasmid

#128722

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcV-hopV1

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP5910

Plasmid

#128717

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcO-hopO1-1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5058

Plasmid

#128710

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcF-hopF2

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-rTH-tTA

Plasmid

#133268

Purpose

An AAV genome that expresses tTA from the rat TH promoter

Depositor

Viviana Gradinaru

Article

Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

rTH

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKM808

Plasmid

#134181

Purpose

Lentiviral sgRNA plasmid with blasticidin resistance

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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