We narrowed to 10,808 results for: T7
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IFI16-(192-393)-HinA
Plasmid#35052DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
TagsHisExpressionBacterialMutationContains HinA domain: aa 192-393PromoterT7Available SinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
C. difficile toxin B mRNA toehold switch sensor
Plasmid#110715PurposeToehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP outputDepositorInsertC. difficile toxin B toehold switch sensor
UseSynthetic BiologyPromoterT7Available SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p42)
Plasmid#135941PurposeExpresses AUF1p42 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
TagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-P5CR
Plasmid#110295PurposeExpresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)DepositorInsertPyrroline-5-carboxylate Reductase
TagsHis6ExpressionBacterialPromoterT7Available SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS1088
Plasmid#80159PurposepET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Cys-4xNuG2-Ald
TagsHisSUMOPromoterT7Available SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30b GluBP600n
Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
TagsHisExpressionBacterialPromoterT7Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlul
Plasmid#119832PurposeProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlul
TagsGSTExpressionBacterialMutationT92APromoterT7Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-ZZ(1700-1751)
Plasmid#99334PurposeBacterial expression of mouse CBP ZZ domainDepositorAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-Myr-FLAG-p110β-DM
Plasmid#55719Purposeeukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224ADepositorInsertp110 beta
Tagsmyristoylation tag (Myr-FLAG)ExpressionMammalianMutationS205D, K224APromoterT7Available SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg2
Plasmid#170329PurposeExpresses RORg isoform 2 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 2 (RORC Human)
TagsHisSUMOExpressionBacterialMutationcodon optimizedPromoterT7Available SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET14b-mEKLF
Plasmid#67834PurposeEncodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitutionDepositorInsertEKLF (Klf1 Mouse)
ExpressionBacterialMutationFull-length mEKLF is aa 20-376; amino acid 19 is …PromoterT7Available SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
Plasmid#61939PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coliDepositorInsertsTags6x HISExpressionBacterialMutationConstruct ends at residue 156PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat
Plasmid#42568DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-At 14-3-3 phi
Plasmid#44939DepositorAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP-HIS-ybbr
Plasmid#153446PurposeE. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.CohE
Tags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only
