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hDNMT3B_KO_puro

Plasmid

#234998

Purpose

Stable knockout of DNMT3B function in mammalian cells

Depositor

Samrat Roy Choudhury

Article

Choudhury et al Genes Dis. 2025 Sep 6;13(1):101843. doi:

Insert

hCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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Scramble_gRNA_puro

Plasmid

#234999

Purpose

scramble sgRNA

Depositor

Samrat Roy Choudhury

Article

Choudhury et al Genes Dis. 2025 Sep 6;13(1):101843. doi:

Insert

scramble sgRNA

Use

Lentiviral

Tags

EGFP:T2A:Puro

Expression

Mammalian

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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Hv_HPT:pGstA+WIR1a:Gus_introns

Plasmid

#215190

Purpose

Evaluating guard cell specific promotors in Barley

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Glutathione-S-transferase1 fused with Wheat‐induced resistance1

Use

Synthetic Biology

Expression

Plant

Promoter

TaGstA1 fused with TaWIR1

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bo_NPTII:pGstA1+WIRa:Gus_introns

Plasmid

#215176

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Glutathione-S-transferase1 Wheat‐induced resistance1

Use

Synthetic Biology

Promoter

TaGstA1 fused with TaWIR1 (chimeric)

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRO204

Plasmid

#233843

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-ABI-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-ABI-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO241

Plasmid

#233844

Purpose

MoClo-YTK; ConLS/R1 cassette; ABA systems; pTDH3-Phlf-NES-PYL1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-Phlf-NES-PYL1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO279

Plasmid

#233845

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-1xOaf1-ABI-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-1xOaf1ABI-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO280

Plasmid

#233846

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-2xOaf1-ABI-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL008

Plasmid

#233849

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN021

Plasmid

#233851

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN057

Plasmid

#233852

Purpose

MoClo-YTK; ConLS/R1 cassette; Auxin systems; pTDH3-AmtR-NES-TIR1-U1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-AmtR-NES-TIR1-U1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN059

Plasmid

#233853

Purpose

MoClo-YTK; ConL1/R2 cassette; Auxin systems; pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN061

Plasmid

#233854

Purpose

MoClo-YTK; ConL1/R2 cassette; NS3 systems; pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN075

Plasmid

#233856

Purpose

MoClo-YTK; ConLS/R1 cassette; Auxin systems; pRPL18B-AmtR-NES-TIR1-U1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-AmtR-NES-TIR1-U1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN076

Plasmid

#233857

Purpose

MoClo-YTK; ConLS/R1 cassette; Auxin systems; pRNR2-AmtR-NES-TIR1-U1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-AmtR-NES-TIR1-U1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN081

Plasmid

#233858

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3a-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NES-NS3a-NLS-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN086

Plasmid

#233859

Purpose

MoClo-YTK; ConLS/R1 cassette; Auxin systems; pTDH3-AmtR-NES-TIR1-U2-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-AmtR-NES-TIR1-U2-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN113

Plasmid

#233862

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-linker-NS3a-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NES-linker-NS3a-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL003

Plasmid

#233864

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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