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Plasmid#37928DepositorInsertEB1 shRNA #1 (MAPRE1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhuman U6Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in pBluescript II
Plasmid#39324DepositorInsertEB1 (MAPRE1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterT7Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mApple-LZ-LOV2
Plasmid#197418PurposeMammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Ï€-EB1_IRES-mCherry
Plasmid#197427PurposeHomology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5DepositorInsertIRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-mCherry
Plasmid#197429PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-EGFP
Plasmid#197430PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsEGFP, GCN4 leucine zipper, LOV2, and Zdk1ExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Top7 Eb13 triple
Plasmid#12475DepositorInsertTop7 Eb13 triple
UseTags6xHisExpressionBacterialMutationEb13 triple. Top7 extended.PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag Raptor Rheb15
Plasmid#26634DepositorInsertraptor-Rheb15 (RPTOR Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorInsertEB1-GFP-6xHis (Mapre1 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-mCherry-6xHis
Plasmid#130984PurposeBacterial expression of mouse EB1-mCherry-6xHis for protein purification.DepositorInsertEB1-mCherry-6xHis (Mapre1 Mouse)
UseTagsmCherry-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-RL-ZEB1 200bmutx5
Plasmid#35537DepositorInsert3'UTR of ZEB1 (ZEB1 Budding Yeast, Human)
UseLuciferaseTagsExpressionMammalianMutation5 mutations in miR200b seed regions of 3'UTR…PromoterAvailable sinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEB1deltaC-2xEGFP shRNA resistant
Plasmid#37925DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationdeletion after aa248, TTCCTGT=>TCCCGGTPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1 fused to monomeric RFP
Plasmid#39323DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in PET28a for protein expression
Plasmid#39297DepositorInsertEB1 (MAPRE1 Human)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag GFP 4E-BP1 Rheb15
Plasmid#112761Purposeexpress GFP-4EBP1-Rheb15DepositorInsertFlag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
UseLentiviralTagsFlag eGFP and Rheb15ExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1
Plasmid#129706PurposeExpress cre-dependent KASH-EGFP and Gmeb1 shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianMutationPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)
Plasmid#72273PurposeFLAG-Mnase expressed under the REB1 promoterDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseTagsExpressionMutationPromoterREB1Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX
Plasmid#112762Purposeexpress GFP-4EBP1-Rheb15(CAAX mutation)DepositorInsertFlag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
UseLentiviralTagsRheb15(CAAX mutant)ExpressionMutationCAAX mutation to prevent lipid localizationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))
Plasmid#39308DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationC-terminal 7 aa (QEEQEEY) removedPromoterCMVAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized Y44 Q46 both converted to A (JB140)
Plasmid#39301DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationY44A and Q46APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized K148 K150 K151 all converted to A (JB144)
Plasmid#39305DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationK148A, K150A and K151APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)
Plasmid#39307DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationT249A, D250A and E251APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized D88 K89 D94 K95 all converted to A (JB141)
Plasmid#39302DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationD88A, K89A, D94A and K95APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)
Plasmid#39303DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationQ109A, W110A, K112A and K113APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized K122 D123 Y124 D125 all converted to A (JB143)
Plasmid#39304DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationK122A, D123A, Y124A and D125APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)
Plasmid#39300DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationS16A, R17A, H18A, D19A, W23A and N25APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)
Plasmid#39306DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationE211A, K212A, E213A, R214A, D215A and Y217APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1
Plasmid#107726PurposeKnock out of EB1 in human cells by CRISPR/Cas9DepositorInsertMAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only