We narrowed to 1,735 results for: nif

Showing: 121 - 160 of 1735 results

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pCW57.1 WT1 +1 ATG

Plasmid

#217643

Purpose

Dox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 long isoform (WT1 Human)

Expression

Mammalian

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-GGA1

Plasmid

#178459

Purpose

Expresses GGA1 in mammalian cells

Depositor

Juan Bonifacino

Article

Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. d

Insert

GGA1 (GGA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

See Depositor Comments Below

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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AP-1 mu1-GFP

Plasmid

#173432

Purpose

Expression of AP-1 Mu1 subunit with a terminal GFP

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP1M1 (Ap1m1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG043

Plasmid

#133123

Purpose

Level 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

PsNifH Synthetic consensus nifH promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo VPS50-13Myc

Plasmid

#175093

Purpose

13Myc tagged VPS50 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

VPS50 (CCDC132 Human)

Tags

13Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEF6 VPS51-V5-6His

Plasmid

#175502

Purpose

V5 tagged VPS51 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

Insert

VPS51 (VPS51 Human)

Tags

V5

Expression

Mammalian

Mutation

A661V- please see depositor comments

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-β4 hinge-ear

Plasmid

#196692

Purpose

Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 β4 hinge-ear (534-739) (AP4B1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 +KTS C terminal HA

Plasmid

#217640

Purpose

Entry vector with WT1 +KTS mutant with C terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 +KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 -KTS C terminal HA

Plasmid

#217642

Purpose

Entry vector with WT1 -KTS mutant with C terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 -KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Mutation

p.408_410delKTS

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 WT1 +378 ATG

Plasmid

#217644

Purpose

Dox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 short isoform (WT1 Human)

Expression

Mammalian

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Ex8 mut WT1

Plasmid

#217645

Purpose

Dox inducible WT1 cDNA with exon 8 mutation

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 (WT1 Human)

Expression

Mammalian

Mutation

Frameshift exon 8

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Ex9 mut WT1

Plasmid

#217646

Purpose

Dox inducible WT1 cDNA with exon 9 mutation

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 (WT1 Human)

Expression

Mammalian

Mutation

Frameshift exon 9

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA2-GAE

Plasmid

#199175

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA2-GAE domain (GGA2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Contains Lys 545 Gln substitution

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA3-GAE

Plasmid

#199176

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA3-GAE domain (GGA3 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA1-GAE

Plasmid

#199174

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA1-GAE domain (GGA1 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGST-parallell2-GGA3(571-723)

Plasmid

#199150

Purpose

Expresses GST-GGA3(571-723) in bacteria

Depositor

Juan Bonifacino

Article

Miller et al Nat Struct Biol. 2003 Aug;10(8):599-606.

Insert

GGA3(571-723) (GGA3 Human)

Tags

GST and TEV protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGAD424-Rabex-5

Plasmid

#197043

Purpose

Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

Rabex-5 (RABGEF1 Bovine)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-AAGAB

Plasmid

#198332

Purpose

Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B-Rigor

Plasmid

#172204

Purpose

Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPN1-ATG9A

Plasmid

#198529

Purpose

Expression of ATG9A-GFP fusion protein in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A (ATG9A Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations in codons 4 and 775

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-RUSH

Plasmid

#202799

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

Lamp1 (Lamp1 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCINeo-myc-Itch (C822S)/2860

Plasmid

#11428

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Itch (Itch Mouse)

Tags

myc

Expression

Mammalian

Mutation

cysteine at 822 mutated to serine

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherry-C-p50

Plasmid

#180238

Purpose

Expresses p50 in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Proc Natl Acad Sci U S A. 2016 Sep 6;113

Insert

p50 (DCTN2 Human)

Tags

pmCherry-C1

Expression

Mammalian

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF1A-Rigor

Plasmid

#172207

Purpose

Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 251 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-(HA)3-ε

Plasmid

#198531

Purpose

Expression of HA-tagged AP-4 ε in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

AP-4 ε (AP4E1 Human)

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitutions in codons 235, 262, 905 and …

Promoter

CMV

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-ε ear

Plasmid

#196670

Purpose

Expresses GST-AP-4 ε ear (residues 840-1137) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε ear (840-1137) (AP4E1 Human)

Tags

GST

Expression

Bacterial

Mutation

silent mutations in codons 905, 1129 and 1137

Promoter

tac

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1-TSF-ε

Plasmid

#196583

Purpose

Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε subunit (AP4E1 Human)

Tags

two Strep/one FLAG (TSF)

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-γ1

Plasmid

#197078

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-1 γ1 (Ap1g1 Mouse)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ATG9A-mCherry

Plasmid

#197393

Purpose

Expresses ATG9A in mammalian cells

Depositor

Juan Bonifacino

Article

De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

Insert

ATG9A (ATG9A Human)

Tags

mCh

Expression

Mammalian

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEP-LAMP1-RFP

Plasmid

#167970

Purpose

Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

Depositor

Juan Bonifacino

Article

Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

LAMP1 (Lamp1 Rat)

Tags

SEP and mRFP

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LAMP1-SBP-GFP

Plasmid

#120172

Purpose

Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Lysosomal-associated membrane protein 1 (Lamp1 Rat)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CD-MPR-GFP-RUSH

Plasmid

#202797

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-(HA)3-ubiquitin

Plasmid

#196991

Purpose

Expresses HA-tagged ubiquitin in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

ubiquitin (UBC Human)

Tags

triple HA tag

Expression

Mammalian

Mutation

silent mutation in ubiquitin codon 13

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-KIF5B*-strep

Plasmid

#120170

Purpose

Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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