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Showing: 121 - 160 of 2387 results

  9. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  10. pCS2-MfnG-EcYRS-EGFP*

    Plasmid
    #191119
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Use
    Tags
    His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191119-plasmid-map-sequence-id-373722

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  11. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    John McCusker
    Insert
    mak10 gRNA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    160385-plasmid-map-sequence-id-384166

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  12. A1-LCD-9F+3Y

    Plasmid
    #178663
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-9F+3Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, three Tyr added
    Promoter
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178663-plasmid-map-sequence-id-356435

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  13. A1-LCD-9F+6Y

    Plasmid
    #178661
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, six Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-9F+6Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, six Tyr added
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178661-plasmid-map-sequence-id-356293

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  14. A1-LCD-8F+4Y

    Plasmid
    #178662
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-8F+4Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    eight Phe residues removed, four Tyr added
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178662-plasmid-map-sequence-id-356201

    Enlarge

  15. A1-LCD-12F+12Y

    Plasmid
    #178659
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD -12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twelve Phe residues replaced with Tyr
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178659-plasmid-map-sequence-id-356187

    Enlarge

  16. A1-LCD+7F-7Y

    Plasmid
    #178660
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD +7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Tyr residues replaced with Phe
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178660-plasmid-map-sequence-id-356154

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  17. A1-LCD Perfect Mix

    Plasmid
    #169718
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. TYR and PHE shuffled into 5 clusters.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169718-plasmid-map-sequence-id-334846

    Enlarge

  18. A1-LCD Aro+

    Plasmid
    #169717
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169717-plasmid-map-sequence-id-334791

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  19. ROR2

    Plasmid
    #39183
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ROR2 (ROR2 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. A1-LCD-30G+30S-12F+12Y

    Plasmid
    #178687
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-30G+30S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser, twelve Phe…
    Promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178687-plasmid-map-sequence-id-357019

    Enlarge

  21. A1-LCD-30G+30S+7F-7Y

    Plasmid
    #178686
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-30G+30S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser, seven Tyr …
    Promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178686-plasmid-map-sequence-id-357017

    Enlarge

  22. A1-LCD-20G+20S+7F-7Y

    Plasmid
    #178688
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, seven Tyr …
    Promoter
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178688-plasmid-map-sequence-id-356407

    Enlarge

  23. A1-LCD-20G+20S-12F+12Y

    Plasmid
    #178689
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, twelve Phe…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178689-plasmid-map-sequence-id-356275

    Enlarge

  24. A1-LCD+23G-23S-12F+12Y

    Plasmid
    #178691
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, twel…
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178691-plasmid-map-sequence-id-356195

    Enlarge

  25. A1-LCD+23G-23S+7F-7Y

    Plasmid
    #178690
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, seve…
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178690-plasmid-map-sequence-id-356145

    Enlarge

  27. pIND4-RC-M210TAG-A7

    Plasmid
    #223015
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosine
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    A7 RS
    pylT tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    223015-plasmid-map-sequence-id-444740

    Enlarge

  28. pJK525

    Plasmid
    #71601
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71601-plasmid-map-sequence-id-131675

    Enlarge

  29. pJK526

    Plasmid
    #71602
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-97 to tyrosine, tryptophan-165…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71602-plasmid-map-sequence-id-131676

    Enlarge

  30. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

    Enlarge

  31. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

    Enlarge

  32. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

    Enlarge

  33. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

    Enlarge

  35. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

    Enlarge

  37. ROR2

    Plasmid
    #39183
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ROR2 (ROR2 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 160 of 2387 results