-
Plasmid#207664PurposeNeuropeptide degradationDepositorInsertNEP (MME Human)
UseAAV and Cre/LoxTagsmRUBY3ExpressionMutationAddition of signaling peptide (POMC) on the N-ter…PromoterhSynapsinAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1-IRES2-EGFP
Plasmid#170820Purposehuman GABAA receptors alpha1 subunit and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1234-1443)
Plasmid#35078DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 1234-1443PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1057-1234)
Plasmid#35076DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 1057-1234PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(936-1113)
Plasmid#35075DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 936-1113PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1113-1316)
Plasmid#35077DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 1113-1316PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(894-1057)
Plasmid#35074DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 894-1057PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-GFP-BRCA1
Plasmid#71116Purposemammalian expression of GFP-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH-SFB-BRCA1
Plasmid#99394PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1DepositorInsertBRCA1 (BRCA1 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-BRCA1
Plasmid#71117Purposemammalian expression of Flag-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV/TOAvailable sinceNov. 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-mCherry-LacR-BRCA1
Plasmid#71115Purposemammalian expression of mCherry-LacR-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF2989
Plasmid#144465PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSLC45A2 (SLC45A2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGRM1-RFP
Plasmid#22920DepositorInserthuman receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(498-663)
Plasmid#35070DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 498-663PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1316-1538)
Plasmid#35079DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 1316-1538PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(540-758)
Plasmid#35071DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 540-758PromoterT7Available sinceMay 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(360-498)
Plasmid#35068DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 498-360PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(758-1057)
Plasmid#35085DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 758-1057PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
RF-118-UB-BRCA1
Plasmid#78960PurposeExpression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBRCA1 (BRCA1 Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-1155PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(663-894)
Plasmid#35072DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 663-894PromoterT7Available sinceMay 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(758-936)
Plasmid#35073DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 758-936PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(408-540)
Plasmid#35069DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 408-540PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(219-758)
Plasmid#35081DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 219-758PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1443-1649)
Plasmid#35080DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 1443-1649PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(170-360)
Plasmid#35066DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 170-360PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF0830
Plasmid#141724PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSLC45A2 (SLC45A2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0829
Plasmid#141723PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSLC45A2 (SLC45A2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1-RNF169UBD
Plasmid#119009PurposeMammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expressionDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1-Rad18UBD
Plasmid#119008PurposeMammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expressionDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(219-408)
Plasmid#35067DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 219-408PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v1
Plasmid#195138Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2475
Plasmid#144337PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFHX3 (ZFHX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1 1366-1437
Plasmid#71118Purposemammalian expession of mCherry-LacR-BRCA1 1366-1437DepositorInsertBRCA1 (BRCA1 Human)
UseTagsmCherryExpressionMammalianMutationcontains amino acids 1366-1437 of BRCA1PromoterCMV/TOAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-GFP-BRCA1 1364-1437
Plasmid#71119PurposeMammalian expression of GFP-BRCA1 1364-1437DepositorInsertBRCA1 (BRCA1 Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 1364-1437 of BRCA1PromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP-BRCA1
Plasmid#115491PurposeEGFP-BRCA1 expression vector. Parton clone BXYDepositorInsertBRCA1 (BRCA1 Human)
UseTagsEGFPExpressionMammalianMutationS1009R (See depositor comments)PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-hPLD3
Plasmid#173851PurposeProtein expression plasmid for recombinant His-Myc tag human PLD3DepositorInsertPLD3 (PLD3 Human)
UseTags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GRM1-DuET
Plasmid#213300PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRM1 (GRM1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GRM1-Tango
Plasmid#66387PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGRM1 (GRM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-BRCA1 3'UTR
Plasmid#29477DepositorInsertBRCA1 3'UTR (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF0058
Plasmid#143007PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAF (MAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0056
Plasmid#142529PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAF (MAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mig P190 (Mig 185)
Plasmid#27483DepositorUseRetroviralTagsGFPExpressionMammalianMutationContains only exon 1 (e1) of bcr fused to ablPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF3282
Plasmid#144758PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBRCA1 (BRCA1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1885
Plasmid#144114PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBRCA1 (BRCA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0057
Plasmid#142530PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAF (MAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1888
Plasmid#144314PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBRCA1 (BRCA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1887
Plasmid#142515PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBRCA1 (BRCA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only