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Showing: 61 - 80 of 17482 results

  6. R6K_tac_GFPmut3_ChInt

    Plasmid
    #187381
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3
    Depositor
    Roi Avraham
    Insert
    GFPmut3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187381-plasmid-map-sequence-id-370413

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  9. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  10. Mut1-RS

    Plasmid
    #182881
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384
    Insert
    Mut1-RS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    182881-plasmid-map-sequence-id-366057

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  12. pHelper_NoMutL_V2_gentR

    Plasmid
    #205293
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    205293-plasmid-map-sequence-id-410127

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  14. Mut2-RS

    Plasmid
    #182882
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384
    Insert
    Mut2-RS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    182882-plasmid-map-sequence-id-366061

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Showing: 61 - 80 of 17482 results