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We narrowed to 2,492 results for: PUC19

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Showing: 61 - 80 of 2492 results

  1. pUC19 (8nt-gap) (NN48)

    Plasmid
    #257526
    Purpose
    To create an 8nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI & Nb.BsmI
    Depositor
    Michael O'Donnell
    Insert
    Nb.BbvCI and Nb.BsmI nicking sites separated by 8 nt
    Use
    Not for gene expression. a dna template for bioch…
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    257526-plasmid-map-sequence-id-512427

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  2. pUC19 (4nt-gap) (NN44)

    Plasmid
    #257524
    Purpose
    To create a 4nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI & Nb.BsmI
    Depositor
    Michael O'Donnell
    Insert
    Nb.BbvCI and Nb.BsmI nicking sites separated by 4 nt
    Use
    Not for gene expression. a dna template for bioch…
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    257524-plasmid-map-sequence-id-512370

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  3. pDD284

    Plasmid
    #66825
    Purpose
    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available Since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  6. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    70684-plasmid-map-sequence-id-306412

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  8. pUC19 (91nt-gap) (NN3)

    Plasmid
    #257528
    Purpose
    To create a 91nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI, Nb.BsmI, and Nt.BspQI. Excised ssDNA are 22-24 bases.
    Depositor
    Michael O'Donnell
    Insert
    Nb.BbvCI, Nb.BsmI, and Nt.BspQI nicking sites
    Use
    Not for gene expression. a dna template for bioch…
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    257528-plasmid-map-sequence-id-512363

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  10. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  12. pUC19-TSV (linearized in LT)

    Plasmid
    #70033
    Purpose
    pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSPyV full genome, linearized in Large T-antigen
    Use
    Cloning vector
    Expression
    Bacterial
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70033-plasmid-map-sequence-id-129217

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  14. pUC19-TSV (linearized in VP2)

    Plasmid
    #70032
    Purpose
    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSV full genome, linearized in VP2 gene
    Use
    Cloning vector
    Expression
    Bacterial
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70032-plasmid-map-sequence-id-129213

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  18. pUC19-SARS-CoV-2-N-gene-truncated

    Plasmid
    #240635
    Purpose
    pUC19-SARS-CoV-2-N-gene-truncated plasmid is a plasmid that contains the SARS-CoV-2 N-gene and can be used as a positive control in SARS-CoV-2 research or diagnostics assays targeting the N gene.
    Depositor
    Kevin Yehl
    Insert
    SARS-CoV-2-N-gene-truncated (N )
    Use
    Synthetic Biology
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    240635-plasmid-map-sequence-id-486094

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Showing: 61 - 80 of 2492 results