We narrowed to 6,239 results for: cas9 expression plasmid

1. pEC1.2/EC1.1_Cas9i_TagRFP

   Plasmid

   #227249

   Purpose

   CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pEC1.2/EC1.1

   Available since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TMPRSS2 g1 + Cas9

   Plasmid

   #153015

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TMPRSS2 g4 + Cas9

   Plasmid

   #153018

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX551-CMV-SpCas9

   Plasmid

   #107024

   Purpose

   AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab

   Depositor

   Alex Hewitt

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEC1.2/EC1.1_spCas9_TagRFP

   Plasmid

   #161934

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEC1.2/EC1.1_spCas9_GFP

   Plasmid

   #161933

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGW_RUBY_EggCas9

   Plasmid

   #225990

   Purpose

   To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas9-NAT

   Plasmid

   #64329

   Purpose

   Cas9 expression cassette carried by a yeast single-copy episome plasmid

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAS9i_TRP

   Plasmid

   #141253

   Purpose

   Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9id_TRP

    Plasmid

    #141254

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACE2 g4 + Cas9

    Plasmid

    #153014

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

    Plasmid Kit

    #1000000210

    Purpose

    Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

    Depositor

    Vincent Martin

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

14. p-dCas9-VP64-Hygro

    Plasmid

    #104405

    Purpose

    transient expression of dCas9-VP64 fusion protein

    Depositor

    Stephan Beck

    Insert

    VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnCas9-AID

    Plasmid

    #196242

    Purpose

    Plasmid expressing the nCas9-AID fusion

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    nickase Cas9 fused to AID
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG1guide_RUBY_EggCas9

    Plasmid

    #225983

    Purpose

    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

    Depositor

    Imran Siddiqi

    Insert

    Guide RNA against AtAGAMOUS1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35s promoter to Drive RUBY and DD45p to drive Cas9

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-SET7-Hygro

    Plasmid

    #104407

    Purpose

    transient expression of dCas9-SET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    SET7 (SETD7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9_mouse_Adar1_ccB

    Plasmid

    #158122

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9_mouse_Adar1_ccA

    Plasmid

    #158120

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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