We narrowed to 4,764 results for: fas

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pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1761 Articles

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pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1761 Articles

Enlarge
pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

697 Articles
pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

697 Articles
GFP-Ub KO.G76V

Plasmid

#11932

Depositor

Nico Dantuma

Publication

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin KO.G76V (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Conjugation deficient mutant. All 7 lysines of ub…

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GFP-Ub KO.G76V

Plasmid

#11932

Depositor

Nico Dantuma

Publication

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin KO.G76V (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Conjugation deficient mutant. All 7 lysines of ub…

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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VCP(wt)-EGFP

Plasmid

#23971

Depositor

Nico Dantuma

Publication

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

none

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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VCP(wt)-EGFP

Plasmid

#23971

Depositor

Nico Dantuma

Publication

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

none

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

Enlarge
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Plasmid

#42335

Purpose

A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Has service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

227 Articles

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Plasmid

#42335

Purpose

A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Has service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

227 Articles

Enlarge
pSIH1-puro-STAT3 shRNA

Plasmid

#26596

Depositor

Frank Sinicrope

Publication

Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

Insert

STAT3 shRNA (STAT3 Human)

Use

Lentiviral and RNAi

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pSIH1-puro-STAT3 shRNA

Plasmid

#26596

Depositor

Frank Sinicrope

Publication

Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

Insert

STAT3 shRNA (STAT3 Human)

Use

Lentiviral and RNAi

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

Enlarge
pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

75 Articles
pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

75 Articles
pAR17_StrepI_hFASh_H8_pET22b

Plasmid

#122846

Purpose

Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Publication

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

human FASN/ human fatty acid synthase (FASN Human)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAR17_StrepI_hFASh_H8_pET22b

Plasmid

#122846

Purpose

Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Publication

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

human FASN/ human fatty acid synthase (FASN Human)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

170 Articles

Enlarge
pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

170 Articles

Enlarge
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

Enlarge
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

Enlarge