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We narrowed to 3,599 results for: fas

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Showing: 61 - 80 of 3599 results

  1. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid
    #122846
    Purpose
    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Publication
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    human FASN/ human fatty acid synthase (FASN Human)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122846-plasmid-map-sequence-id-238539

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  6. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42335-plasmid-map-sequence-id-275689

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  10. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid
    #48141
    Purpose
    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid
    #122846
    Purpose
    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Publication
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    human FASN/ human fatty acid synthase (FASN Human)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122846-plasmid-map-sequence-id-238539

    Enlarge

  12. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid
    #48140
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48140-plasmid-map-sequence-id-334358

    Enlarge

  15. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42335-plasmid-map-sequence-id-275689

    Enlarge

  17. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid
    #48141
    Purpose
    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  18. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid
    #62987
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62987-plasmid-map-sequence-id-435258

    Enlarge

  19. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid
    #48140
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48140-plasmid-map-sequence-id-334358

    Enlarge

  20. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42333
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    March 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42333-plasmid-map-sequence-id-59273

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Showing: 61 - 80 of 3599 results