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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,107 results for: gibson assembly

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Showing: 61 - 80 of 1107 results

  4. p006-GFP-pBAD

    Plasmid
    #108315
    Purpose
    GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    GFP under control of arabinose-inducible promoter pBAD
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    108315-plasmid-map-sequence-id-218821

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  11. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  12. The Xenorhabdus griffiniae part library

    Plasmid Kit
    #1000000274
    Purpose
    Use this parts library for Golden Gate and Gibson assemblies to express DNA parts in the bacterium Xenorhabdus griffiniae.
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Feb. 13, 2026
    Availability
    Academic Institutions and Nonprofits only

  18. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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Showing: 61 - 80 of 1107 results