270,538 results

1. pBABE GFP

   Plasmid

   #10668

   Purpose

   Empty backbone for retroviral gene expression. Select recipient cells using GFP.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHSE401

   Plasmid

   #62201

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHSE401

   Plasmid

   #62201

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-mDlx-GFP-Fishell-1 (AAV PHP.eB)

   Viral Prep

   #83900-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Promoter

   mDlx

   Tags

   GFP

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

5. pET-21b(+)-Is-PETase-W185A

   Plasmid

   #112204

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W185A

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-21b(+)-Is-PETase-W185A

   Plasmid

   #112204

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W185A

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn5-145

   Plasmid

   #118529

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn5-145

   Plasmid

   #118529

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM90

   Plasmid

   #133647

   Purpose

   Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-SS-mCherry-HDEL
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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