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pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PFA0445w-bio-His

Plasmid

#50811

Purpose

Expresses enzymatically monobiotinylated full length PFA0445w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0445w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF10_0166-bio-His

Plasmid

#50801

Purpose

Expresses enzymatically monobiotinylated full length PF10_0166

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF10_0166

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-rat Fcepsilon RI gamma

Plasmid

#8375

Depositor

Jean Kinet

Article

Blank et al Nature 1989 Jan 12;337(6203):187-9.

Insert

rat Fc epsilon RI gamma cDNA (Fcer1g Rat)

Use

Beta-gal

Expression

Bacterial

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
HOXD13 (chicken) HIS-tag pET

Plasmid

#8555

Depositor

Corey Largman

Article

Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

Insert

HOXD13 (HOXD13 )

Tags

His and T7

Expression

Bacterial

Mutation

contains 10 nt 5' to ATG start; makes prote…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
vsv-Sgo1 N61I-GFP

Plasmid

#108497

Purpose

expression of vsv-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C/T180C

Plasmid

#66182

Purpose

Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C, T180C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C/L171C

Plasmid

#66178

Purpose

Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C, L171C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR 19b-3p

Plasmid

#82442

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-19-3p

Mutation

Complementary region for hsa-miR-19-3p as sensor

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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Dom34(265-386)

Plasmid

#19102

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S1215X)

Plasmid

#73970

Purpose

STAG2 cDNA (CCDS43990) S1215X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1215X

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (Q1117X)

Plasmid

#73968

Purpose

STAG2 cDNA (CCDS43990) Q1117X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

Q1117X

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S1075X)

Plasmid

#73967

Purpose

STAG2 cDNA (CCDS43990) S1075X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1075X

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S653X)

Plasmid

#73965

Purpose

STAG2 cDNA (CCDS43990) S653X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S653X

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pST39-His/Vps27(345-440) - Hse(275-375)

Plasmid

#17638

Depositor

James Hurley

Article

Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

Insert

His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pBacPAK8-His-VHL-Flag(117-213)

Plasmid

#29501

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Tags

Flag and His

Expression

Insect

Mutation

VHL(117-213) only

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pET16b-Trx80

Plasmid

#36945

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Trx80 (truncated Trx) (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

premature stop inserted after K80 in Trx1 gene

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pBJ1 neo-mo FceRI beta

Plasmid

#8604

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI beta cDNA (Ms4a2 Mouse)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-ZO-2-1st-PDZ

Plasmid

#27424

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only