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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAXEF-Rap1-E63(GTP)

Plasmid

#32698

Depositor

Vicki Boussiotis

Article

Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

Insert

RAP1 (RAP1A Human)

Expression

Mammalian

Mutation

E63: Consitutively GTP-bound

Promoter

EF promoter

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#192871

Purpose

Gateway compatible vector with BsaI cloning site

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Available Since

Dec. 12, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Duet1-His-TEV-mCherry-p62

Plasmid

#199780

Purpose

Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

6x His, TEV, mCherry

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBDK

Plasmid

#135190

Purpose

Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

KanR

Use

Synthetic Biology

Expression

Bacterial

Promoter

cat

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pYpet-PBD

Plasmid

#22781

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-SYFP2

Plasmid

#189762

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN PLEKHM1-HA

Plasmid

#89300

Purpose

For expression of PLEKHM1 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pARF6-YFP

Plasmid

#11389

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

YFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pKD233.7-3

Plasmid

#90955

Purpose

Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23114, PltetO-1, and PttrB185-269

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pQCi-mB2m-sgRNA

Plasmid

#154090

Purpose

pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting murine B2M locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-mBAF45c IRES GFP

Plasmid

#17882

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45c (Dpf3 Mouse)

Tags

FLAG and IRES EGFP

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only
4xHRE_minCMV-sfGFP-CMV_dsRed Exp

Plasmid

#117401

Purpose

sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159873

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-PBD

Plasmid

#11394

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 (PAK1 Human)

Tags

CFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCyPet-Rac1(WT)

Plasmid

#22782

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (wt) (RAC1 Human)

Tags

CyPet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P41nmt1-mECitrine

Plasmid

#105152

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

kanMX6-P41nmt1-mECitrine

Tags

41nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

41nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hGFAP-Pink Flamindo-WPRE-SV40

Plasmid

#178790

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

Pink Flamindo

Use

AAV

Promoter

hGFAP

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCfb12620

Plasmid

#219928

Purpose

PrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TPI

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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