We narrowed to 10,082 results for: crispr plasmids

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFP-KASH

Plasmid

#154373

Purpose

Vector for Cre-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-tmpknot-GG-acceptor

Plasmid

#174039

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD

Plasmid

#235574

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPP537

Plasmid

#158803

Purpose

pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDD428

Plasmid

#202081

Purpose

Cas9/sgRNA plasmid targeting the C-terminus of Myh9

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-u6-gRNA(deltaD1)-hSyn-mCherry

Plasmid

#231400

Purpose

Knockdown of DRD1 across rodent species

Depositor

Arjen Boender, Larry Young

Article

Karkare et al bioRxiv [Preprint]. 2025 Oct 21:2025.10.

Insert

sgRNA(DRD1.2)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-u6-gRNA(deltaD2)-hSyn-mCherry

Plasmid

#231401

Purpose

Knockdown of DRD2 across rodent species

Depositor

Arjen Boender, Larry Young

Article

Karkare et al bioRxiv [Preprint]. 2025 Oct 21:2025.10.

Insert

sgRNA(DRD2.1)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAS088

Plasmid

#211502

Purpose

AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Type

Empty backbone

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-TET1CD

Plasmid

#184439

Purpose

TET1 catalytic domain fused with sfGFP and scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

scFv-GCN4-sfGFP-TET1CD

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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EBOV 4cis

Plasmid

#249332

Purpose

Integration plasmid encoding EBOV essential replication factors (NP, VP35, VP30, and L protein)

Depositor

Amy Kistler

Insert

EBOV NP-P2A-VP35-P2A-VP30-P2A-L protein

Use

Piggybac donor vector

Expression

Bacterial and Mammalian

Mutation

the EBOV NP, VP35, and VP30 ORF sequences within …

Promoter

CAG

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCK255

Plasmid

#171155

Purpose

Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

I-SceI_sacB

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMC3-TPK2

Plasmid

#176180

Purpose

yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2 (TPK2 Budding Yeast)

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAT9658-sgRNA-mCherry

Plasmid

#162987

Purpose

sgRNA cloning plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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