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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,774 results for: BLI;

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Showing: 8021 - 8040 of 9774 results

  1. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  2. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid
    #221358
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221358-plasmid-map-sequence-id-436819

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  5. DT480

    Plasmid
    #80413
    Purpose
    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80413-plasmid-map-sequence-id-151399

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  8. Tandem p40p35

    Plasmid
    #108665
    Purpose
    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
    Depositor
    Nevil Singh
    Insert
    interleukin-12 (Il12a, Il12b Mouse)
    Expression
    Mammalian
    Mutation
    IL-12 dimers have a Gly-Ser linker
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    108665-plasmid-map-sequence-id-260875

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  9. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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  13. pc DNA FRT TO- APPSwed/Ind

    Plasmid
    #114194
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    Popular mouse model, overexpress 2 mutations: Swe…
    Promoter
    CMV
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114194-plasmid-map-sequence-id-224256

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  14. eGFP-hCOL1A1

    Plasmid
    #140110
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag, K20…
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140110-plasmid-map-sequence-id-276323

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  15. HaloTag-beta2-adrenergic receptor

    Plasmid
    #66994
    Purpose
    Beta-2-adrenergic receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-beta2-adrenergic receptor (ADRB2 Human)
    Tags
    Human Growth hormone Signal Sequence-HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66994-plasmid-map-sequence-id-115414

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  16. pCMV-Spike-VLP

    Plasmid
    #173922
    Purpose
    Expresses virus-like particles based upon the original SARS-Cov-2
    Depositor
    Andreas Stuermer
    Inserts
    SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 E protein (E SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 M Protein (M SARS-COV-2, Human, Synthetic)
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Promoter
    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…
    Available Since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173922-plasmid-map-sequence-id-358853

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  17. pAG mKappa Anti-c-Myc [9E10]

    Plasmid
    #242956
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse kappa light chain constant region; to be used with pAG mIgG2a Anti-c-Myc [9E10] heavy chain (Plasmid 242955) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242956-plasmid-map-sequence-id-485246

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  18. pLJM1 EGFP-Tau

    Plasmid
    #108868
    Purpose
    Expresses EGFP linked to human Tau
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    human four-repeat tau lacking the N-terminal sequ…
    Promoter
    CMV
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108868-plasmid-map-sequence-id-237064

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  19. NanoBRET Assay Vector, NanoLuc-KRas(G12C)

    Plasmid
    #238582
    Purpose
    Express NanoLuc-KRas(G12C) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238582-plasmid-map-sequence-id-478460

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Showing: 8021 - 8040 of 9774 results