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pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pJC-TALE-hairy

Plasmid

#46145

Depositor

David Stern

Article

Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

Type

Empty backbone

Tags

Hairy Repression Domain

Expression

Insect

Promoter

hsp70

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCVII-BFP

Plasmid

#162755

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mTagBFP2

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9_sgRNA_expression_in_pBluescript

Plasmid

#122089

Purpose

U6 driven SpCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAW

Plasmid

#64745

Purpose

Yeast expression plasmid: ACC1

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1

Expression

Yeast

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTAL-MajSat-FLAG-NLS-MCS

Plasmid

#69073

Purpose

Expresses TALE for mouse major satellite with FLAG tag under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-mTFP1

Plasmid

#49644

Purpose

TALETeal-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

mTFP1

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pHRCMV 14-3-3 sigma

Plasmid

#16552

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

14-3-3 sigma (SFN Human)

Use

Adenoviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Frb-NLS-mCherry

Plasmid

#217613

Purpose

part B of SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

Frb-NLS-mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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HA-RNF126

Plasmid

#138647

Purpose

Mammalian expression of 2HA-tagged RNF126

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Tags

HA-HA

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP26-1A(sfGFP)

Plasmid

#126973

Purpose

Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato

Plasmid

#125573

Purpose

Cre-dependent expression of tdTomato in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z28

Plasmid

#120071

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P29

Plasmid

#120040

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pME nls-eos

Plasmid

#73791

Purpose

Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

Depositor

David Raible

Article

Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

Insert

nls-eos

Use

Middle entry clone

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only