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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,931 results for: nin

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Showing: 8041 - 8060 of 66931 results

  1. pCAG MCP-VP64

    Plasmid
    #92357
    Purpose
    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP-VP64
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92357-plasmid-map-sequence-id-178154

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  2. pKD184

    Plasmid
    #90953
    Purpose
    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively
    Depositor
    Jeffrey Tabor
    Publication
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    Expression
    Bacterial
    Promoter
    Bba_J23114, PltetO-1, and PphsA
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90953-plasmid-map-sequence-id-178324

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  3. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70703-plasmid-map-sequence-id-127765

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  8. pX459-sgAAVS1

    Plasmid
    #184403
    Purpose
    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT
    Depositor
    Robert Bradley
    Publication
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-specific sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184403-plasmid-map-sequence-id-362381

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  12. DD-DamV133A-IRES2-mCherry

    Plasmid
    #159600
    Purpose
    Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.
    Depositor
    Aaron Streets
    Publication
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-DamV133A-IRES2-mCherry
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159600-plasmid-map-sequence-id-311948

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  19. pUF1-dCas9-GCN5

    Plasmid
    #122509
    Purpose
    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Publication
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GCN5
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122509-plasmid-map-sequence-id-245888

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Showing: 8041 - 8060 of 66931 results