We narrowed to 9,676 results for: BLI

1. SUMO CRYGS 16-177 Q16C

   Plasmid

   #217673

   Purpose

   N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

   Depositor

   Larry David

   Insert

   CRYGS (CRYGS Human)
   

   Tags

   6XHis and SUMO

   Expression

   Bacterial

   Mutation

   Glutamine 16 changed to Cysteine, and amino acids…

   Promoter

   T7

   Available Since

   May 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMamCol1

   Plasmid

   #215184

   Purpose

   Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

   Depositor

   Krishna Kishore Inampudi

   Insert

   tsPurple
   

   Use

   AAV, CRISPR, and Synthetic Biology ; Recombinant …

   Tags

   SNAC-StrepII tag

   Expression

   Bacterial and Mammalian

   Promoter

   TetON 3G Bidirectional for Mammalian and Trc with…

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GAL4(1-147)

   Plasmid

   #195029

   Purpose

   The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   GAL4 (1-147)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GL4.10 Prg4 promoter-luciferase

   Plasmid

   #195034

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Prg4 promoter (PRG4 Bovine)
   

   Use

   Luciferase

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-T7-hTP53-P0~670-FLuc

   Plasmid

   #208640

   Purpose

   for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.

   Depositor

   Quanfu Ma

   Insert

   TP53, UTR and partial CDS (TP53 Human)
   

   Use

   Luciferase; In vitor transcription template

   Tags

   Firefly Luciferase (F-Luc) and T7 promoter

   Mutation

   UTR and partial CDS in-frame with F-Luc

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

   Plasmid

   #208645

   Purpose

   Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
   

   Use

   CRISPR; Ssdna donnor preparation for knock-in of…

   Tags

   Twin Strep

   Mutation

   Two SNP (rs749555789, rs760194863) were introduce…

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWR54

   Plasmid

   #203330

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Expression

   Yeast

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWR24

   Plasmid

   #202771

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coNAT
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Expression

   Yeast

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWR16

   Plasmid

   #202711

   Purpose

   integrating bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   URA3 and flanking sequence
   

   Expression

   Yeast

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid

    #200389

    Purpose

    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    PX458_3XHA_dFnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid

    #203805

    Purpose

    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    Llgl1 (Llgl1 Mouse)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid

    #196973

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    GFP and GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

    Plasmid

    #196976

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

    Depositor

    Lukas Kapitein

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    GST and SNAP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-SU(Z)12(624-900)-H6

    Plasmid

    #183772

    Purpose

    Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

    Insert

    SU(Z)12 residues 624-900 (Su(z)12 Fly)
    

    Tags

    His tag at the C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid

    #105635

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid

    #105637

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+WBP2NL-HA

    Plasmid

    #174791

    Purpose

    Xenopus laevis WBP2NL with 3'HA tag

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    WBP2 N-terminal like (wbp2nl.L Frog)
    

    Tags

    HA

    Expression

    Bacterial

    Mutation

    aa#165, Y to H; aa# 287, D to H

    Promoter

    SP6

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR4 CDS A. thaliana PARP1

    Plasmid

    #175818

    Purpose

    pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Insert

    PARP1 (PARP1 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Mutation

    one silent mutation, annotated in GenBank file

    Promoter

    none

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+-wbp2nl

    Plasmid

    #172463

    Purpose

    expresses wild-type protein when injected into embryos

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nl (wbp2nl.L Frog)
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid

    #168770

    Purpose

    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Mutation

    See Depositor Comments Below

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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