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Plasmid#141604PurposeLentiviral vector for overexpressing the ZNF3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF3 (ZNF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0403
Plasmid#141605PurposeLentiviral vector for overexpressing the ZNF3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF3 (ZNF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE027
Plasmid#176784PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert304
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pKdH_P-GAP-ScACS1*
Plasmid#126736Purposeyeast plasmid overexpressing ACS1 from Saccharomyces cerevisiaeDepositorInsertacs1*
UseTags6xHis,MycExpressionYeastMutationT3542C and A30S, P113S, and S629N (please see dep…PromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104A
Plasmid#125826PurposeExpresses GST-RPN13 (19-132) R104A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged arginine 104 to alaninePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104D
Plasmid#125827PurposeExpresses GST-RPN13 (19-132) R104D in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged arginine 104 to aspartatePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104E
Plasmid#125828PurposeExpresses GST-RPN13 (19-132) R104E in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged arginine 104 to glutamatePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shGDF11 #2
Plasmid#83084PurposeLentiviral shRNA vector for inducible knockdown of human GDF11DepositorInsertshGDF11 (GDF11 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]
Plasmid#106466PurposeExpresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Serine 219 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]
Plasmid#106467PurposeExpresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(H474A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Histidine 474 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]
Plasmid#106468PurposeExpresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(D415A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Aspartate 415 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-401
Plasmid#89866PurposeExpresses human BROX residues 1-401 in bacteria; internal ID WISP11-297DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationexpresses only residues 1-401PromoterT7Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
PAN3 gRNA (BRDN0001146404)
Plasmid#77423Purpose3rd generation lentiviral gRNA plasmid targeting human PAN3DepositorInsertPAN3 (PAN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAN3 gRNA (BRDN0001146135)
Plasmid#77424Purpose3rd generation lentiviral gRNA plasmid targeting human PAN3DepositorInsertPAN3 (PAN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAN3 gRNA (BRDN0001147703)
Plasmid#77425Purpose3rd generation lentiviral gRNA plasmid targeting human PAN3DepositorInsertPAN3 (PAN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAN3 gRNA (BRDN0001146176)
Plasmid#77426Purpose3rd generation lentiviral gRNA plasmid targeting human PAN3DepositorInsertPAN3 (PAN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXJ42-p200 CUX1
Plasmid#100813PurposePlasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tagDepositorInsertp200 CUX1 (CUX1 Human)
UseNote that this plasmid does not have an sv40 oriTagsHA and MycExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14-GINIP
Plasmid#204845PurposeExpress 3x FLAG tagged-GINIP in mammalian cellsDepositorInsertPHF24 (KIAA1045 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
Plasmid#71294PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGNSTM glycosylation motif, HA, Lamp2 signal pepti…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-LAMP2
Plasmid#222321PurposeSynchronize the trafficking of LAMP2 from the ER.DepositorInsertStreptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRTagsExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
H11-mScarletSIIN
Plasmid#172438PurposeHipp11 targeting vector, expresses mScarlet-SIINFEKLDepositorInsertmScarlet-SIIN
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2388
Plasmid#144020PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF462 (ZNF462 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1526
Plasmid#144182PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXI1 (MXI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPH2
Plasmid#19535DepositorInsertheat shock 70kDa protein 4 (HSPA4 Human)
UseTagsHisExpressionMammalianMutationmutations A381T, T732A, and an insertion of "…PromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF1527
Plasmid#143128PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXI1 (MXI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-EMTB-TurboID-V5
Plasmid#190736PurposeMammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)DepositorInsertensconsin (MAP7 Human)
UseTagsMyc, TurboID, and V5ExpressionMammalianMutationMicrotubule binding-domain of ensconsin (amino ac…PromoterCMVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-HADH2
Plasmid#67863Purposebacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)DepositorInsertHADH2 (HSD17B10 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only