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pISceI Dusp6:D2EGFP

Plasmid

#32667

Depositor

Michael Tsang

Article

Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E3

Plasmid

#15789

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pTOX12

Plasmid

#127457

Purpose

Encodes Tse2 toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTOX1

Plasmid

#127446

Purpose

Encodes YhaV toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTOX2

Plasmid

#127447

Purpose

Encodes MqsR toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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His-lsMSP1D1

Plasmid

#240151

Purpose

Expression of the MSP 'His-lsMSP1D1'

Depositor

Lise Arleth

Article

Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

Insert

His-lsMSP1D1

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Promoter

SP6

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAd5-B1

Plasmid

#122551

Purpose

Block 1 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Promoter

None

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGH941

Plasmid

#230846

Purpose

Ef1a-DivA-BE-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pFGM1

Plasmid

#64949

Purpose

source of GmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-GmR-FRT

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-chmI

Plasmid

#221341

Purpose

Express ChmI in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmI

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_HA

Plasmid

#184866

Purpose

pSwap plasmid part with HA homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST17-UbE2L3

Plasmid

#15795

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pTOX11

Plasmid

#127456

Purpose

Encodes MqsR toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2-eNOSPromoter-Luciferase

Plasmid

#19297

Depositor

Charles Lowenstein

Article

Arnet et al J Biol Chem. 1996 Jun 21. 271(25):15069-

Insert

endothelial nitric oxide synthase promoter (NOS3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pTOX10

Plasmid

#127455

Purpose

Encodes YhaV toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2S

Plasmid

#15807

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2S (UBE2S Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pAVEC0_N-mEGFP

Plasmid

#231255

Purpose

For expressing fusion proteins with N terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MyrPalm-CKAR

Plasmid

#14862

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSWAP_Entry_H2_beta_GM

Plasmid

#184871

Purpose

pSwap plasmid part with H2_beta homology arms and gentamycin cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCS2+CyclinB1-GFP

Plasmid

#128426

Purpose

Contains insert allowing in-vivo visualization of CyclinB1 protein (G2/M)

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

CyclinB1-GFP

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pU6-As-crRNA

Plasmid

#78956

Purpose

Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBPGUw-HACK-G4>QF2

Plasmid

#80277

Purpose

GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKm-HCBE

Plasmid

#170333

Purpose

a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized CBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-HBx

Plasmid

#24931

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

EGFP and HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_C-mEGFP

Plasmid

#231263

Purpose

For expressing fusion proteins with C terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_N-mScarletI

Plasmid

#231274

Purpose

For stably expressing fusion proteins with N terminally tagged mScarletI in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMetOYC-Dest

Plasmid

#105082

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

Depositor

Christopher Grefen

Article

Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

Type

Empty backbone

Tags

Cub-PLV and mOST4

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CFP-CaaX

Plasmid

#155232

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

CFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Tags

CFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-3xGFP-tDeg

Plasmid

#191371

Purpose

Overexpression of 3xGFP-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

3xGFP-tDeg

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2A

Plasmid

#15780

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2A (UBE2A Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2T

Plasmid

#15808

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2T (UBE2T Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2B

Plasmid

#15781

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2B (UBE2B Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2C

Plasmid

#15779

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2C (UBE2C Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-Lck-mScarlet3

Plasmid

#234540

Purpose

to label the membrane of cells with a fluorescent protein (mScarlet3)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mScarlet3

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
His-lsMSP1E3D1

Plasmid

#240152

Purpose

Expression of the MSP 'His-lsMSP1E3D1'

Depositor

Lise Arleth

Article

Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

Insert

His-lsMSP1E3D1

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2G1

Plasmid

#15790

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pCiVSP-SD64TF

Plasmid

#80332

Purpose

Expresses Ciona VSP in Xenopus Oocyte

Depositor

Yasushi Okamura

Article

Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

Insert

Ciona intestinalis voltage-sensing phosphatase (vsp Ciona intestinalis)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1C

Plasmid

#139470

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

stuffer

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
piRFP682-N1

Plasmid

#45459

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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