We narrowed to 2,005 results for: ENDOG

1. pGEMHE-mEGFP-Map4

   Plasmid

   #105571

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   MAP4 (Map4 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCB15

   Plasmid

   #194756

   Purpose

   GluRIIA-Rescue-Construct for integration at attP sites

   Depositor

   Sven Dannhäuser

   Article

   Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

   Insert

   GluRIIA (GluRIIA Fly)
   

   Use

   Phic31-integration vector

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAC93-pmax-dCas9VP160

   Plasmid

   #48225

   Purpose

   dCas9VP160 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV_NOPlight1

   Plasmid

   #195578

   Purpose

   Expresses NOPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

   Insert

   NOPLight1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Circular 100,50 (RAB7A)

   Plasmid

   #170117

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 100,50 guide RNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Circular 200,100 with interspersed loops (SMAD4)

   Plasmid

   #180191

   Purpose

   AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEMHE-mCherry-mTrim21ΔPRYSPRY

   Plasmid

   #105524

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Expression

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

   Plasmid

   #112157

   Purpose

   Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

   Depositor

   Ben Giepmans

   Article

   de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

   Insert

   anti-GFP FLIPPER-body
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAC2-dual-dCas9VP48-sgExpression

   Plasmid

   #48236

   Purpose

   Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9VP48
   

   Use

   CRISPR

   Tags

   HA-tag and VP48

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-PITCh-MYO1C

    Plasmid

    #227305

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFJ150-GFP(dpiRNA)::CDK-1

    Plasmid

    #107938

    Purpose

    cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    cdk-1

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC153-dual-dCas9VP96-sgExpression

    Plasmid

    #48239

    Purpose

    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP96

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-C-Solo-mStayGold-PXN

    Plasmid

    #227316

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold Tag (PXN Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-FLEX-FLIM-AKART391A

    Plasmid

    #60446

    Purpose

    Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKART391A
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 391 to Ananine

    Promoter

    CAG

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

    Plasmid

    #47457

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Use

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-45b-mEGFP

    Plasmid

    #185013

    Purpose

    Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    Sirius-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC91-pmax-dCas9VP64

    Plasmid

    #48223

    Purpose

    dCas9VP64 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEMHE-mCherry-mTrim21ΔRING-Box

    Plasmid

    #105523

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139485

    Purpose

    PCR template for Halo-Tag gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    HaloTag-loxP-P2A-dTKneo-pA-loxP
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-C-Solo-mScarlet-H2BC11

    Plasmid

    #207756

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge