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pG20_TEV_Flag_FR

Plasmid

#240668

Purpose

Expression plasmid for TEV-Flag-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-Flag

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet-larA-strep-larB

Plasmid

#232792

Purpose

Expression of L. plantarum larA-strep and larB genes in E. coli

Depositor

Robert Hausinger

Article

Nevarez et al mBio. 2024 Dec 16:e0340424. doi: 10.1128

Inserts

Lactate racemase
Pyridinium-3,5-biscarboxylic acid mononucleotide synthase

Tags

Strep-tag II

Expression

Bacterial

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_K217R/K327R

Plasmid

#237511

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_K217R/K327R

Expression

Plant

Mutation

Mutations at kinase active sites (K217R, K327R)

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_Reg2

Plasmid

#237513

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg2

Expression

Plant

Mutation

Removed binding region 2

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-PKD

Plasmid

#237521

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_pseudokinase

Expression

Bacterial

Mutation

Pseudokinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca+C-Tail

Plasmid

#237526

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Expression

Yeast

Mutation

Add C-terminal extension

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-KD

Plasmid

#237520

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_kinase

Expression

Bacterial

Mutation

Kinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_Reg1

Plasmid

#237512

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Plant

Mutation

Removed binding region 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-GFP-hCGNL1

Plasmid

#236521

Purpose

For expression of Paracingulin (human) in insect cells

Depositor

Sandra Citi

Article

Flinois et al MicroPubl Biol. 2024 Oct 14;2024:10.1791

Insert

CGNL1 (CGNL1 Human)

Tags

2xStrep-10xHis-Tev-EGFP

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca+C-Tail

Plasmid

#237514

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Expression

Plant

Mutation

Add C-terminal extension

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL678

Plasmid

#207511

Purpose

GmUbi3-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmUBI3

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL679

Plasmid

#207512

Purpose

GmM4-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL681

Plasmid

#207514

Purpose

GmM4-SpRY ABE-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-sgRNA006_CTCF

Plasmid

#232928

Purpose

Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9 (Ctcf S. pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-AviTag-CC-S2

Plasmid

#233243

Purpose

Bacterial expression of Avi-CC-S2

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

AviTag-CC-S2

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-larE-larC

Plasmid

#232793

Purpose

Expression of L. plantarum larE and LarC genes in E. coli

Depositor

Robert Hausinger

Article

Nevarez et al mBio. 2024 Dec 16:e0340424. doi: 10.1128

Inserts

Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein

Expression

Bacterial

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-5xQUAS::Δpes-10P::AI::gur-3G

Plasmid

#232613

Purpose

Expression of 'GUR-3' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::QF+hGR::SL2::tagBFP

Plasmid

#232607

Purpose

Pan-neuronal expression of chimeric protein 'QF+hGR' and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

QF+hGR

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-5xQUAS::Δpes-10P::AI::prdx-2G

Plasmid

#232614

Purpose

Expression of 'PRDX-2' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

PRDX-2 (prdx-2 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DiO-eGFP-2A-mCherry_YDA

Plasmid

#194881

Purpose

Ratiometric YDA sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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