We narrowed to 16,015 results for: grn

1. pNOC-CRISPR-GFP

   Plasmid

   #100009

   Purpose

   Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-GFP

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-Cetn1/1

   Plasmid

   #50718

   Purpose

   pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Inserts

   Cetn1 sgRNA1
   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and hU6

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFYF1548 EMX1

   Plasmid

   #47508

   Purpose

   human gRNA expression vector targeting EMX1

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EMX1 (EMX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCE41

   Plasmid

   #174433

   Purpose

   C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SiC-V1

   Plasmid

   #133041

   Purpose

   SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGCP123-EbpA_g1

   Plasmid

   #153517

   Purpose

   sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGCP123-GFP_g1

   Plasmid

   #153518

   Purpose

   sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV_AA089

   Plasmid

   #216105

   Purpose

   gRNA expression for Cas12a (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gRNA expression
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGTRm

   Plasmid

   #127197

   Purpose

   pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA PCR template
   

   Use

   Template plasmid for generation of golden gate pc…

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-eCas9-sgNT

    Plasmid

    #140238

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-RRBP1-1

    Plasmid

    #92156

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJZC43

    Plasmid

    #66565

    Purpose

    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-UGCG-KO

    Plasmid

    #80010

    Purpose

    gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    UGCG (UGCG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYDE007

    Plasmid

    #194152

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1333

    Plasmid

    #119268

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Carol Gross, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. sg14x(MS2) MUC4.1

    Plasmid

    #101153

    Purpose

    gRNA with 14 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1187

    Plasmid

    #119256

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Carol Gross, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-eCas9-sgMIB1

    Plasmid

    #140239

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    sgRNA targeting human MIB1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiGuide-RRBP1-2

    Plasmid

    #92157

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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