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pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_X

Plasmid

#147991

Purpose

Insect Expression of DmNot1-del1156-1169-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

Plasmid

#147875

Purpose

Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

Plasmid

#147859

Purpose

Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL1-COMP5AP-AviTag-9xHis

Plasmid

#157232

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL1 (KIR2DL1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VSIG2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156659

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSIG2 (VSIG2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2_1-496-siR

Plasmid

#196515

Purpose

Expression of Myc-Eps8L2_1-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

Plasmid

#195495

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-2 PX459 plasmid

Plasmid

#192256

Purpose

expressing sgRNA-2 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-1 PX459 plasmid

Plasmid

#192255

Purpose

expressing sgRNA-1 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-1 PX459 plasmid

Plasmid

#192253

Purpose

expressing sgRNA-1 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-2 PX459 plasmid

Plasmid

#192254

Purpose

expressing sgRNA-2 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-2 PX459 plasmid

Plasmid

#192250

Purpose

expressing sgRNA-2 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-1 PX459 plasmid

Plasmid

#192249

Purpose

expressing sgRNA-1 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166133

Purpose

This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting cldn1 gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166134

Purpose

This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting firefly luciferase gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-Truncated-Kir3.2-StreptagII

Plasmid

#177263

Purpose

Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

Depositor

Arthur Laganowsky

Article

Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

Insert

G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)

Tags

StreptagII

Expression

Insect

Mutation

Residues 49-378

Promoter

polyhedrin promoter

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KHBD00021

Plasmid

#34183

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG14548 (E(spl)mbeta-HLH Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6c

Plasmid

#207853

Purpose

Mammalian expression of PE6c prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6c

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCE-K2N

Plasmid

#154879

Purpose

Episomally expresses KLF2 and NANOG in mammalian cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Insert

KLF2-2A-NANOG (KLF2, NANOG Human)

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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