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FHUG+W (B2)

Plasmid

#74011

Purpose

lentiviral expression of EGFP and shRNA (from H1 promoter, empty)

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGST-PPARalpha

Plasmid

#16550

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR alpha (PPARA Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal DNA-binding domain: aa 1-249

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pACS45

Plasmid

#204581

Purpose

GST-lamin A (389-646)

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

lamin A (389-646)

Tags

GST - lamin A (389-646)

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 EGFP-Sumo2 GG

Plasmid

#13384

Depositor

Mary Dasso

Article

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 GG (SUMO2 Human)

Tags

EGFP and GST

Expression

Bacterial

Mutation

Mature SUMO2GG: diglycine at amino acid positions…

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pSiren_EGFP-shLuc

Plasmid

#122287

Purpose

Knockdown control

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for Luc

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-HBH-kanMX6

Plasmid

#26872

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pPL5617_pUG_PuroR

Plasmid

#60928

Purpose

PCR template vector for generating puromycin resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

puromycin N-Acetyltransferase

Use

Loxp flanked deletion cassette template

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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eNOS S1179D

Plasmid

#22484

Depositor

William Sessa

Article

Fulton et al Nature. 1999 Jun 10. 399(6736):597-601.

Insert

eNOS S1179D

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
PBS/pU6-HIF1 alpha RNAi plasmid 2

Plasmid

#21104

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

RNAi against hypoxia inducible factor 1alpha

Expression

Mammalian

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pTHE

Plasmid

#12512

Depositor

Tong-Chuan He

Article

Jiang et al J Biol Chem. 2001 Nov 30. 276(48):45168-

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pMOTag-glmS-4H

Plasmid

#106378

Purpose

Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS-3xHA

Available Since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-EF1a-Backbone(NN)

Plasmid

#27961

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NN)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc

Plasmid

#10972

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
lvl0 alcSynth-34S

Plasmid

#153411

Purpose

Ethanol system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

alcSynth-34S promoter

Expression

Plant

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVimentin-PSmOrange2

Plasmid

#34961

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Tags

Vimentin

Expression

Mammalian

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pKB222

Plasmid

#170012

Purpose

Encodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
LL-37

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-ESYN-R5

Plasmid

#32581

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Enhanced Synapsin Promoter

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pTXG

Plasmid

#52280

Purpose

Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Type

Empty backbone

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJA327

Plasmid

#74486

Purpose

superfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

superfolder GFP

Expression

Worm

Promoter

myo-3

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sizzled

Plasmid

#24684

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Expression

Mammalian

Available Since

July 22, 2010

Availability

Academic Institutions and Nonprofits only