167,785 results

1. pRN114

   Plasmid

   #127221

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, Wus, AtSTM
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-CMV-polysite

   Plasmid

   #122843

   Purpose

   Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #169283

   Purpose

   Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

   Depositor

   Dirk Heckl

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hfCas12Max

   Plasmid

   #195336

   Purpose

   vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized hfCas12Max
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   N243R, E336R, D892R

   Promoter

   CBh, CMV, hU6

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

   Plasmid

   #216156

   Purpose

   Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with C-terminal FLAG tag
   

   Expression

   Mammalian

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 FRT TO GFP11-LRRK2_G2019S

   Plasmid

   #231175

   Purpose

   Expresses GFP-11-LRRK2 G2019S in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   GFP11-LRRK2 (G2019S) (LRRK2 Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   G2019S

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP12689 - pAAV-AiE0682h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2689)

   Plasmid

   #191727

   Purpose

   AI plasmid ALIAS: AiP12689. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   none

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. COC053

   Plasmid

   #209131

   Purpose

   Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

   Depositor

   Christopher A. O'Callaghan

   Article

   O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

   Insert

   Enzyme BirA (birA E. coli)
   

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+mScarlet3-C Cloning Vector

   Plasmid

   #220616

   Purpose

   To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   mScarlet3 fusion

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid

    #107581

    Purpose

    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-IRF9

    Plasmid

    #71452

    Purpose

    Lentiviral expression of IRF9

    Depositor

    George Stark

    Article

    Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

    Insert

    IRF9 (IRF9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-FLAG-S6K1

    Plasmid

    #100509

    Purpose

    Expresses FLAG tagged S6K1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-BP1-2 pLPC-Puro

    Plasmid

    #19835

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

    Insert

    Fragment of p53-Binding Protein 1 (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Mutation

    Fragment of human 53BP1 aa 1220 - 1711

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. AMPK-SPARK

    Plasmid

    #226255

    Purpose

    phases-of-separation based activity reporter of AMPK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK-SPARK
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. FTO-dCas9

    Plasmid

    #134781

    Purpose

    Expression fusion protein FTO-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    FTO and inactive dCas9
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-HA-CDK4

    Plasmid

    #172612

    Purpose

    Expresses HA-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+)/FaGH16A

    Plasmid

    #100910

    Purpose

    Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FaGH16A
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Vector 1174D

    Plasmid

    #200012

    Purpose

    Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

    Depositor

    Omar Akbari

    Article

    Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

    Insert

    Engineered dobulesex splicing module
    

    Use

    Synthetic Biology

    Expression

    Insect

    Mutation

    Engineered dobulesex splicing module

    Promoter

    Hr5IE1

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HC078 (3xHA-Atg13)

    Plasmid

    #59544

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Tags

    3xHA

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pAAVone-AAV-PHP.eB-CMV-eGFP

    Plasmid

    #230929

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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