We narrowed to 15,727 results for: NOL

1. CABE-T3.1

   Plasmid

   #193267

   Purpose

   Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T3.1
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. rnLbCas12a-PE2

   Plasmid

   #213746

   Purpose

   For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   rnLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, H759A, R1138A in LbCas12a

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA304

   Plasmid

   #216072

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.minBG-CI-SaCas9-W3SynpA

   Plasmid

   #231369

   Purpose

   minBG-driven SaCas9, with short synthetic terminator sequence.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   SaCas9
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.minCMVe-SCP1-SaCas9-W3SynpA

   Plasmid

   #231370

   Purpose

   SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   SaCas9
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG sHRPa-FRB-pre mGRASP

   Plasmid

   #73145

   Purpose

   Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-FRB-pre mGRASP
   

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

   Plasmid

   #160611

   Purpose

   Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:LbCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. [pSK467] pLJC5 HA-Depdc5

   Plasmid

   #109343

   Purpose

   Lenti-viral expression of HA-tagged wildtype Depdc5

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB_OTmut

   Plasmid

   #185388

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Has Service

   AAV1 and AAV9

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaTCH BC1

    Plasmid

    #154195

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    CaTCH BC1
    

    Use

    Lentiviral; Catch barcode labeling

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CaTCH_empty-backbone

    Plasmid

    #157746

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral; Catch barcode cloning

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA031

    Plasmid

    #216011

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpRY (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AA030

    Plasmid

    #216010

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpG (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-ms-Sox17

    Plasmid

    #50781

    Purpose

    retroviral expression of mouse Sox17

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox17 (Sox17 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PresentER-NLVPMVATV (GFP)

    Plasmid

    #102947

    Purpose

    PresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Cancer Immunol Res. 2020 May;8(5):672-68

    Insert

    NLVPMVATV
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD2529-CAG-HA-mouse GARP

    Plasmid

    #180258

    Purpose

    Mouse full length GARP expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    GARP (Lrrc32 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-OsTIR1(F74A)

    Plasmid

    #140731

    Purpose

    OsTIR1(F74A)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74A)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid

    #191164

    Purpose

    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (additional cutsites)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9 TadCBEa

    Plasmid

    #193782

    Purpose

    Express TadCBEa (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa-SpCas9D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-Cluc-RMA

    Plasmid

    #189624

    Purpose

    Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    Cypridina luciferase fused to Fc
    

    Use

    AAV and Luciferase

    Tags

    Cluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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