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pFUW-tetO-Zbtb46

Plasmid

#139811

Purpose

doxycycline-inducible expression of mouse Zbtb46 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Zbtb46 (Zbtb46 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs_FLAG-ODC1

Plasmid

#133256

Purpose

Express FLAG-tagged ODC1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

ODC1 (ODC1 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pG4-E6s-luc

Plasmid

#46324

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

5xGal4

Use

Luciferase

Tags

E boxes and luciferase

Expression

Mammalian

Mutation

five copies of a multimerized GAL4 (5×GAL4) site …

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pXGFPC-5 Pxyl-dcas9 (Spy)

Plasmid

#133316

Purpose

expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus pyogenes)

Use

CRISPR

Promoter

xylose

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-EPA-DNNNS-DQNRT

Plasmid

#128397

Purpose

In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A

Tags

6xHis tag

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK_Zeo_3xflag_Luciferase

Plasmid

#136533

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

Luciferase

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJB001 CMV 3xFLAG-NLS-FKBP-ZF2

Plasmid

#161545

Purpose

Constitutive expression of 3xFLAG-NLS-FKBP-ZF2 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-FKBP-ZF2

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB003 ZF1/2x6-C 3xFLAG-NLS-VP16-FRB

Plasmid

#161546

Purpose

Inducible expression of 3xFLAG-NLS-VP16-FRB under the ZF1/2x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP16-FRB

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

ZF1/2x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJKW1813

Plasmid

#174003

Purpose

Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP90G4
ATR1 (ATR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC12)_Psyn-sgRNArodA

Plasmid

#149661

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC12), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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UKNP1.21.2

Plasmid

#98200

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN2

Plasmid

#110636

Purpose

Retroviral expression of Flag-SFXN2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN2 (SFXN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

Plasmid

#149588

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN4

Plasmid

#110638

Purpose

Retroviral expression of Flag-SFXN4 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN4 (SFXN4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET23a-MmRPS27a

Plasmid

#69559

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human, Mouse)

Expression

Bacterial

Mutation

Changed Alanine 133 (human RPS27a) to Glycine (mo…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pWB-MF-IKKe

Plasmid

#44116

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

IKKe (IKBKE Human)

Use

Retroviral

Tags

Myristoylated Flad

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pSPYngK-hp

Plasmid

#48118

Purpose

Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

signal peptide of YngK

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

Plasmid

#192609

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS-P2A-tagBFP

Use

Lentiviral and Synthetic Biology

Tags

tagBFP

Promoter

PGK

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1_V591A_S602D

Plasmid

#48190

Purpose

Expresses V591A and S602D mutated Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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