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Showing: 8361 - 8380 of 8817 results

  1. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  3. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid
    #118412
    Purpose
    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.
    Depositor
    Nicole Paulk
    Article
    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1
    Insert
    Firefly luciferase
    Use
    AAV, Luciferase, and Synthetic Biology
    Expression
    Insect and Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118412-plasmid-map-sequence-id-231800

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  11. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Article
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Available Since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    196196-plasmid-map-sequence-id-385065

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  16. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

    Plasmid
    #111394
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    GCaMP6f
    Use
    AAV
    Tags
    6xHis, Myc, T7, and Xpress
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    111394-plasmid-map-sequence-id-212394

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  18. pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL

    Plasmid
    #111397
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON hM4Di-mCherry (Gi-coupled DREADD for neuronal silencing), W3SL cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    hM4Di-mCherry
    Use
    AAV
    Tags
    Myc and mCherry
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    111397-plasmid-map-sequence-id-212158

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  20. TFORF1777

    Plasmid
    #144310
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WHSC1 (NSD2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    144310-plasmid-map-sequence-id-471158

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Showing: 8361 - 8380 of 8817 results