We narrowed to 9,955 results for: FIE

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pEF/myc/ER FLII12Pglu-700uDelta6

Plasmid

#17867

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pBLO1806_Cas9_2xNLS_human

Plasmid

#74493

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-hsp70

Plasmid

#71500

Purpose

flySTARR-seq screening vector with hsp70 core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1662

Plasmid

#51482

Purpose

miniMos transposable element with HygroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-sfGFP

Plasmid

#166126

Purpose

sfGFP cre reporter

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

sfGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr-Mef2aflbio

Plasmid

#32970

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Mef2a (MEF2A Human)

Use

Gateway

Tags

flag-bio

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pME-mKate2-NS

Plasmid

#109729

Purpose

Multisite gateway vector for 5' tagging with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Setd3-Flag-pcDNA3.0

Plasmid

#49144

Purpose

mammalian expression of Flag tagged Setd3

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-MCS-BioID2-3xFLAG

Plasmid

#186812

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ1663

Plasmid

#51484

Purpose

miniMos transposable element with HygroR and peel-1, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-FAF1S181G

Plasmid

#107987

Purpose

expression of oncogenic FAF1

Depositor

Georg Weber

Article

Weber et al Sarcoma. 2015;2015:839182. doi: 10.1155/

Insert

FAF1S181G (FAF1 Human)

Expression

Mammalian

Mutation

carcinogenic mutant, S181G

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-GFP

Plasmid

#40123

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-p40R57Q PX

Plasmid

#19009

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

aa1-148 (PX domains) site directed mutagenesis: …

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ1655

Plasmid

#51481

Purpose

miniMos transposable element with HygroR, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSH9

Plasmid

#90328

Purpose

Expresses the set-2.a cDNA isoform in the C. elegans intestine

Depositor

Anne Brunet

Article

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

Pges-1::set-2a

Expression

Worm

Promoter

ges-1

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-eSpCAS9-NG-pA

Plasmid

#131466

Purpose

Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized eSpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-T1ER

Plasmid

#47928

Purpose

T1ER is a calcium sensor targeted to ER

Depositor

Tobias Meyer

Article

Bandara et al Sci Signal. 2013 Jul 9;6(283):ra56. doi:

Insert

T1ER

Expression

Mammalian

Mutation

KDEL

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pBB14

Plasmid

#18657

Depositor

Lynn Enquist

Article

Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

Insert

Us9-EGFP gene fusion

Tags

EGFP

Expression

Mammalian

Mutation

First 106 amino acids of Us9 are intact. The stop…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR-Rabin8

Plasmid

#24910

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rabin8 (RAB3IP Human)

Use

Gateway entry vector

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pLBH546_Tet-Cas14b1Locus

Plasmid

#112503

Purpose

Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLBH547_Tet-Cas14b2Locus

Plasmid

#112504

Purpose

Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b2

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab33bb in pCSDEST2

Plasmid

#140882

Purpose

Expression of zebrafish GFP-tagged Rab33bb in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab33bb

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-SpCAS9-NG-pA

Plasmid

#131465

Purpose

Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized SpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-rBcan

Plasmid

#18958

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican (Bcan Rat)

Expression

Mammalian

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-neo-Strep_Flag_Nterm

Plasmid

#102644

Purpose

Epitope tag n-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al J Biol Chem. 2016 Sep 2;291(36):18818-42

Type

Empty backbone

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-DAT-myc

Plasmid

#107457

Purpose

Mammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Dopamine Transporter (DAT) (SLC6A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L

Plasmid

#52481

Purpose

Expresses mCherry, EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pMH183

Plasmid

#122856

Purpose

MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-modified sgRNA targeting the Arabidopsis FT promoter

Use

Golden gate compatible cloning vector

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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