We narrowed to 8,753 results for: lif
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Plasmid#158769PurposeiGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.DepositorInsertiGluSnFR
UseLentiviralMutationAdditional ER and Golgi export sequencesAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_( E3_5)_FLAG
Plasmid#199612PurposeEncodes DARPin-FLAG E3_5 for bacterial expression and column purificationDepositorInsertDARPin-FLAG E3_5 (control)
Tags8xHis and FLAGExpressionBacterialPromoterT5Available SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK66
Plasmid#176606PurposeTo measure Syk intensity in response to ligand clustering using DNA origamiDepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
P442
Plasmid#139728PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsiveDepositorInsertpMOD_D_pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromoterAtRAB18Available SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-Cre
Plasmid#122562PurposeIntegration of Cre sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-Cre-SV40pA
UseCloningAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLIP38
Plasmid#65616PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edCit-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3
Plasmid#122092PurposeAAV plasmid for expressing rsCaMPARI in neurons, nucleus localizedDepositorInsertrsCaMPARI
UseAAVTagsNLS-His and mRuby3Promoterhsyn (synapsin-1)Available SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
P223
Plasmid#139720PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, CPHDepositorInsertpMOD_A'_pLHB1B1:NnCPH:tSlH4
UseLuciferaseExpressionPlantPromoterAtLHB1B1Available SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA (KmR)
Plasmid#102321Purposedrives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)DepositorInsertRpaA with p0050 promoter (+ kanamycin resistance casette)
UseCloning vectorPromoter-Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP43
Plasmid#65618PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-BFLF1
Plasmid#162626PurposeExpresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cellsDepositorInsertBFLF1
TagsN-terminal 2xStrep-Tag IIExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLIP37
Plasmid#65505PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-Cer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn CCL20-pre-mGRASP
Plasmid#173117PurposeExpresses CCL20 sender construct in neuronsDepositorInsertCCL20-pre-mGRASP
UseAAVPromoterSynapsinAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Paxillin LD
Plasmid#186150PurposeFor expressing recombinant paxillin variants in bacteria.DepositorInsertPXN (PXN Human)
TagsGSTExpressionBacterialMutationD5A; D146A; D232A; D267A; D301APromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Paxillin
Plasmid#186143PurposeFor expresseion of recombinant paxillin in bacteriaDepositorInsertPXN (PXN Human)
TagsGSTExpressionBacterialMutationPaxillin C1997A (silent mutation for cloning)PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3195
Plasmid#172695PurposePunc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)DepositorInsertPunc-25
TagsGFP 1-10ExpressionWormAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::RpaA D53A
Plasmid#105229Purposeinducible expression of unphosphorylatable variant of RpaA (D53A)DepositorInsertrpaA driven by Ptrc promoter
UseCyanobacteria cloning vectorMutationD53 mutated to APromoterptrcAvailable SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Char-BFP2-TSERex
Plasmid#124792PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertChar-BFP2
ExpressionMammalianPromoterCAGAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseExpressionPlantPromoterAtuMASAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w
Plasmid#162481PurposeA bicistronic construct - FloxGal80 and UAS-mCD8GFPDepositorInsertCre80Tom-GFP
UseCre/LoxAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK33/intein-Cas9_S219-3XFLAG
Plasmid#133782PurposeExpresses human codon-optimized intein-Cas9DepositorInsertHuman codon-optimized intein-Cas9
ExpressionInsectAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx4
Plasmid#104474PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
TagsFKBPx4-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP154
Plasmid#106360PurposeA vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.DepositorTypeEmpty backboneExpressionWormAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LexAop2->Bxb1.STOP>myr::4xSNAPf
Plasmid#87640PurposeFor creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPfDepositorInsertmyr::4xSNAPf
ExpressionInsectAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP30
Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonr201_PARG WT
Plasmid#240315PurposeEntry vector for Gateway with PARGDepositorInsertPARG (PARG Human)
ExpressionBacterialAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJFT7_nHalo_DC(r4)_PARG_Mutation
Plasmid#240316PurposeGateway compatible vector expressing PARG (E755/756A)DepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEE-moxBFP-IRES-PuroR
Plasmid#242413PurposeSpectral unmixing: moxBFP onlyDepositorInsertsmoxBFP
IRES-PuroR
UseSelf-amplifying rnaExpressionMammalianAvailable SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-PuroR
Plasmid#242415PurposeSpectral unmixing: mScarlet3 onlyDepositorInsertsmScarlet3
IRES-PuroR
UseSelf-amplifying rnaExpressionMammalianAvailable SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
RNF168 C-terminal sgRNA
Plasmid#207100PurposepX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.DepositorInsertGAGATGCACAAAGTAAGGCC
ExpressionMammalianPromoterCMV and U6Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
RIF C-terminal sgRNA
Plasmid#207096PurposepX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.DepositorInsertAATACTAAATAGAATTTTCA
ExpressionMammalianPromoterCMV and U6Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
REV7 C-terminal sgRNA
Plasmid#207095PurposepX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.DepositorInsertGCTCATAAAGGCAGCTGAGG
ExpressionMammalianPromoterCMV and U6Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-BoxB-petracrRNA
Plasmid#207625PurposeExpresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)DepositorInsertBoxB-petracrRNA
UseSynthetic BiologyPromoterhU6Available SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM198
Plasmid#227657PurposepRS305 His-HRV3C-Rpn5 WTDepositorAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM200
Plasmid#227659PurposepRS305 3XFLAG-HRV3C-Rpn5 WTDepositorAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDestTol2-QUAS:GFP-CAAX-he1.1:YFP
Plasmid#184816PurposeUsed to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic lineDepositorInsertQUAS:GFP-CAAX-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsGFP-CAAXAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDR274-gsc2-sgRNA
Plasmid#184818PurposeUsed to synthesize gRNA targeting exon 2 of the gsc2 geneDepositorInsertgsc2-sgRNA
UseCRISPR; Template for sgrna synthesisAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only