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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(TAL1(11))-PGKpuro2ABFP-W

Plasmid

#208545

Purpose

Lentiviral vector expressing gRNA targeting human TAL1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

TAL1(11) (TAL1 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NDUFA1(11))-PGKpuro2ABFP-W

Plasmid

#200494

Purpose

Lentiviral vector expressing gRNA targeting human NDUFA1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

NDUFA1(11) (NDUFA1 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

Plasmid

#52093

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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HSC1-HS4-D4Z4c-GiP

Plasmid

#58541

Purpose

Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab11 DN

Plasmid

#37712

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S25N (dominant negative)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGG-PIP39

Plasmid

#196777

Purpose

Contains WRKY11 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 11 (WRKY11 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

240 C->G

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Expression

Mammalian

Promoter

Pgk

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXTK123 [Misc_Expanding-Modulator-B_PartType8-11]

Plasmid

#229360

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Misc_Expanding-Modulator-B_PartType8-11

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

Expanding Modulator B (sfGFP)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK125 [Misc_Terminating-Modulator-B_PartType8-11]

Plasmid

#229362

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Misc_Terminating-Modulator-B_PartType8-11

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

Terminating Modulator B (sfGFP)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGWT35S-VC155

Plasmid

#194051

Purpose

Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pNP159

Plasmid

#106361

Purpose

To insert a single copy of a construct at a site on Chr II, corresponding to GFP-labelled RAB-11 expressed specifically in the epidermis.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Insert

ttTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2+pCmYLCV911

Plasmid

#170878

Purpose

Phytobrick - pCmYLCV911

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pCmYLCV911

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (T56D)

Plasmid

#24237

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (S58D)

Plasmid

#24239

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pGWT35S-AtOEP7(1-50aa)-VC155

Plasmid

#194046

Purpose

Transient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Tags

AtOEP7 1–50aa

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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NHA-Delta 11

Plasmid

#36926

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 722-941 only

Available Since

May 24, 2012

Availability

Academic Institutions and Nonprofits only
EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab11 CA

Plasmid

#53472

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Tags

YFP

Expression

Insect

Mutation

Q70L

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only