We narrowed to 4,740 results for: MLS

1. pLV-REPORT(EF1a)

   Plasmid

   #172326

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFYF1548 EMX1

   Plasmid

   #47508

   Purpose

   human gRNA expression vector targeting EMX1

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EMX1 (EMX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. D. vulgaris sp2 CRISPR/pACYCDuet-1

   Plasmid

   #81186

   Purpose

   Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
   

   Use

   CRISPR

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZLblasti-CTR1

   Plasmid

   #53157

   Purpose

   Expresses human CTR1 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

   Plasmid

   #182825

   Purpose

   Cre-dependent expression of H2B-APEX fusion

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   H2B.APEX-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GOMO-GFP

   Plasmid

   #60975

   Purpose

   Rapid selection of recombinant marker-free fluorescent rodent malaria parasites

   Depositor

   Olivier Silvie

   Article

   Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

   Inserts

   GFP
   mCherry
   hDHFR-yFCU
   

   Use

   Plasmodium gene targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. DT041A

   Plasmid

   #159402

   Purpose

   Constitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancer

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pLenti-CMV-TrkB (Ntrk2, Mouse) (Ntrk2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET19b-EcMscL-TEV-mEGFP

   Plasmid

   #165097

   Purpose

   Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

   Depositor

   Neha Kamat

   Article

   Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

   Insert

   E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
   

   Use

   Synthetic Biology

   Tags

   monomeric Enhanced Green Fluorescent Protein

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFPZFN1.4-B2H

    Plasmid

    #26791

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

11. GFPZFN2-B2H

    Plasmid

    #26792

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pLV-U-TVA950

    Plasmid

    #115236

    Purpose

    Lentiviral transfer plasmid for expression of TVA950

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TVA950
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF208wt-luc

    Plasmid

    #122143

    Purpose

    For luciferase expression

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Fam208a promoter (Tasor Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fam208a

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVT/Smarca4

    Plasmid

    #122146

    Purpose

    For making Smarca4 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarca4 (Smarca4 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 14x PP7 with loxP-kanMX-loxP

    Plasmid

    #189939

    Purpose

    Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

    Depositor

    Tineke Lenstra

    Article

    Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

    Insert

    14x PP7 with loxP-kanMX-loxP
    

    Use

    Tags

    kanMX

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

    Plasmid

    #182824

    Purpose

    Cre-dependent expression of cytosolic APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    APEX.NES-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAIP

    Plasmid

    #74171

    Purpose

    Lentiviral vector for expression of heterologous genes

    Depositor

    Jeremy Luban

    Article

    Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS ColI alpha2

    Plasmid

    #19207

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Alpha 2 type I collagen (COL1A2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pAM5080

    Plasmid

    #85106

    Purpose

    Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    YFP-KaiC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

    Plasmid

    #182826

    Purpose

    Cre-dependent expression of membrane localized APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    LckAPEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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