We narrowed to 5,561 results for: donor

1. pD(Gib)_3'delta

   Plasmid

   #223869

   Purpose

   4G-cloning donor for 3'-Gibson overhang, delta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'delta sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-DRGFP

   Plasmid

   #113193

   Purpose

   DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

   Depositor

   Adam Karpf

   Insert

   DR-GFP
   

   Use

   Donor construct with t2a-hygromycin for gene trap…

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-hSox2

   Plasmid

   #49389

   Purpose

   donor vector for cloning human Sox2 with 6xHN tag

   Depositor

   Randall McKinnon

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Cre/Lox; Donor vector

   Tags

   6xHN (6 repeating His-Asn subunits)

   Mutation

   TGA (stop) to CCA (Pro) at nt 1027 with respect t…

   Promoter

   none

   Available Since

   Nov. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pD(P+T)_CMVN

   Plasmid

   #223861

   Purpose

   4G-cloning donor for mammalian promoter and terminator, N-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   CMV promoter and bGH polyA
   

   Use

   Golden-gate donor

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD(P+T)_phN

   Plasmid

   #223858

   Purpose

   4G-cloning donor for insect promoter and terminator, N-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   polyhedrin promoter and SV40 polyA
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD(Ct)_3C-TS-His10

   Plasmid

   #223893

   Purpose

   4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-TwinStrep-His10
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD(Ct)_Avi-3C-TS

   Plasmid

   #223894

   Purpose

   4G-cloning donor for C-terminal AviTag-3C-TwinStrep tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   AviTag-3C-TwinStrep
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD(Nt)_His6-GST-TEV

   Plasmid

   #223878

   Purpose

   4G-cloning donor for N-terminal His6-GST-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-GST-TEV
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD(Nt)_His6-MBP-TEV

   Plasmid

   #223877

   Purpose

   4G-cloning donor for N-terminal His6-MBP-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-MBP-TEV
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD(Nt)_His6-HA2-TEV

    Plasmid

    #223876

    Purpose

    4G-cloning donor for N-terminal His6-HA2-TEV tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-HA2-TEV
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD(Nt)_His6-FLAG3-TEV

    Plasmid

    #223875

    Purpose

    4G-cloning donor for N-terminal His6-FLAG3-TEV tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-FLAG3-TEV
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD(P+T)_phC

    Plasmid

    #223859

    Purpose

    4G-cloning donor for insect promoter and terminator, C-terminal tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    polyhedrin promoter and SV40 polyA
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD(P+T)_T7N

    Plasmid

    #223855

    Purpose

    4G-cloning donor for bacterial promoter and terminator, N-terminal tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    T7 promoter and terminator
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD(P+T)_T7C

    Plasmid

    #223856

    Purpose

    4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    T7 promoter and terminator
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD(Nt)_His10-TS-3C

    Plasmid

    #223884

    Purpose

    4G-cloning donor for N-terminal His10-TwinStrep-3C tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His10-TwinStrep-3C
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD(Ct)_3C-Venus-His8

    Plasmid

    #223889

    Purpose

    4G-cloning donor for C-terminal 3C-Venus-His8 tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    3C-Venus-His8
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD(P+T)_CMVC

    Plasmid

    #223862

    Purpose

    4G-cloning donor for mammalian promoter and terminator, C-terminal tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    CMV promoter and bGH polyA
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mouse 3' Ubf AID GFP PuroR

    Plasmid

    #127901

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene

    Depositor

    Mark Groudine

    Insert

    Ubf (Ubtf Mouse, Mustard Weed)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Ubf

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. BFP dest clone

    Plasmid

    #71825

    Purpose

    Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. EasyFusion Halo

    Plasmid

    #112850

    Purpose

    General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    HaloTag
    

    Use

    Synthetic Biology

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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