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pBBR1_DVK_EF

Plasmid

#188947

Purpose

Level 1 destination vector containing pBBR1 origin of replication and CIDAR EF scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_FG

Plasmid

#188948

Purpose

Level 1 destination vector containing pBBR1 origin of replication and CIDAR FG scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_GH

Plasmid

#188949

Purpose

Level 1 destination vector containing pBBR1 origin of replication and CIDAR GH scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVS_AG

Plasmid

#188951

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AG scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVS_AH

Plasmid

#188952

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AH scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVC_AF

Plasmid

#188953

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AF scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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T7-5iX

Plasmid

#106991

Purpose

T7 transcription template for 5iX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

5iX

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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T7-Xi3

Plasmid

#106992

Purpose

T7 transcription template for Xi3

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xi3

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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T7-3eX

Plasmid

#106993

Purpose

T7 transcription template for 3eX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

3eX

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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T7-Xe5

Plasmid

#106994

Purpose

T7 transcription template for Xe5

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xe3

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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T7-5i3eX

Plasmid

#106996

Purpose

T7 transcription template for 5i3eX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

5i3eX

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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T7-Xi3e5

Plasmid

#106997

Purpose

T7 transcription template for Xi3e5

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xi3e5

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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T7-Xi3eX

Plasmid

#106998

Purpose

T7 transcription template for Xi3eX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xi3eX

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2/25Q

Plasmid

#1384

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (25Q) (HTT Human)

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-GFP

Plasmid

#159654

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Syk

Plasmid

#50045

Purpose

Expression of mCherry-Syk in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Spleen tyrosine kinase (Syk) (Syk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnai1

Plasmid

#245996

Purpose

Express HA N-terminal tagged mouse Galphai1 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnai1 (Gnai1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186WT-HA

Plasmid

#239774

Purpose

Expresses WT rat NF186 with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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